Project Details
Description
Ethyl alcohol (henceforth ethanol) is a human carcinogen.1,2 Its consumption has been associated with cancers
at various sites, including the oral cavity. Despite strong epidemiological evidence, the mechanisms of ethanol
carcinogenicity remain unclear, hampering the ability to develop efficacious preventive strategies, identify
individual susceptibility, and effectively face the challenges deriving from the projected increase in consumption.
Ethanol major metabolite, acetaldehyde (AA), is suggested to play a crucial role in head and neck cancers by
reacting with DNA. These reactions generate chemical modifications (DNA adducts) that, if not repaired, may
result in mutations and ultimately lead to cancer. Individuals with genetic deficiencies in ALDH2, the enzyme
responsible for AA detoxification, were shown to have a 15% increased risk of developing oral cancer when
drinking.5 Additionally, Fanconi Anemia (FA) patients, who have impaired mechanisms to repair AA-related DNA
damage, have an average 500-fold higher chance of developing oral cancer.6 Previous studies have shown a
direct and dose-dependent connection between ethanol consumed and AA-derived DNA damage, in the oral
cavity of healthy volunteers.7 This effect was not as evident in blood DNA from the same individuals, indicating
a distinct contribution of AA exposure coming from oral ethanol metabolism by the mucosa and oral microbiome.
Our hypothesis is that AA resulting from oral metabolism of ethanol is playing a crucial role in oral cancer
through the formation of DNA adducts, and that levels and persistence of driver adducts will increase in
individuals with increasing oral cancer risk. Using cutting-edge analytical approaches, our objective is to
characterize ethanol's oral metabolism and its corresponding DNA damage and mutational profiles, to develop
a systematic assembly of biomarkers for identifying oral cancer risk and for developing strategies for early
detection and prevention. This will be done by completing 3 aims. The first one will characterize DNA damage
profiles in oral cells, collected after exposure to a controlled alcohol dose from participants from 3 groups at
increasing risk of AA-related oral cancer (active ALDH2*1/1* homozygotes, inactive ALDH2*1/2* heterozygotes
and FA patients). Driver adducts will be identified as those increasing in the groups following the increased
cancer risk and persisting over time in FA patients. The second aim will focus on investigating the role of the oral
microbiome in ethanol metabolism, by characterizing the oral microflora and measuring the aldehyde profile
resulting from the ethanol dose in the saliva of study participants. Additionally, the oral microbiome will be
characterized in samples from non-drinkers, included to investigate if ethanol consumption results in a specific
profile. Finally, the third aim will analyze genome-scale mutational signatures in oral cell lines exposed to AA, for
which DNA adducts will be profiled, and in oral tumor tissues from rats chronically exposed to AA or ethanol.
This will yield mechanistic evidence on ethanol-related oral mutagenesis and cancer formation and identify DNA
adducts bearing mutagenic potential.
Status | Active |
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Effective start/end date | 9/20/22 → 8/31/24 |
Funding
- National Institute on Alcohol Abuse and Alcoholism: $540,286.00
- National Institute on Alcohol Abuse and Alcoholism: $489,210.00
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