Project Details
Description
PROJECT SUMMARY
Associating stimuli in the environment to biologically relevant outcomes, such as reward or threat, is necessary
to survive and foundational to decision making. Given the prevalence of disordered decision making, there is a
pressing need to understand the basic neurobiological mechanisms of associative learning underlying
cue-guided motivation and behavior. The Ventral Tegmental Area (VTA) is essential to this behavioral process
through its two main neuronal subtypes: dopamine (DA) and GABA. GABA neurons synapse directly onto local
DA neurons and modulate DA transmission. DA neurons increase activity to cue-reward associations,
modulating firing based on the extent to which reward predicted matches reward expected in a phenomenon
known as reward prediction error (RPE). GABA neurons also increase activity in reward learning and their
signaling contributes to RPE DA dynamics. In contrast, DA neurons are inhibited in aversive contexts while
GABA neurons increase activity. Given VTA GABA’s role in both appetitive and aversive processing, these
neurons may be uniquely engaged to integrate valence in decision making. Understanding the role of these
neurons in multi-valent learning is important, because behavior often takes place in situations of motivational
conflict, where opposing goals (i.e., consuming food and avoiding threats) occur simultaneously, requiring the
appetitive and aversive elements to be weighed and integrated to guide choices. This proposal will make
use of new tools to target, record, and manipulate VTA DA and GABA neurons, to investigate
their functional connectivity (Aim 1) and their roles in valence integration (Aim 2). First, I will
optogenetically inhibit GABA neurons while recording the activity of VTA DA neurons through in vivo fiber
photometry. These experiments will test the hypothesis that the VTA GABA modulates local DA neurons and
this relationship can change with experience. I will also manipulate and record DA and GABA dynamics during
a motivational conflict task in which there are two opposing goals (consuming sucrose and avoiding shock) to
dissect the roles these populations play in valence integration. These studies will test the hypothesis that VTA
DA and GABA neurons produce value and salience signals, respectively, that are collectively necessary to
integrate valence, for dynamic reward seeking.
Status | Active |
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Effective start/end date | 9/1/23 → 8/31/24 |
Funding
- National Institute on Drug Abuse: $34,953.00
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