δ-opioid receptors inhibit neurogenic intestinal secretion evoked by mast cell degranulation and type I hypersensitivity

Sutthasinee Poonyachoti; David R. Brown

doi:10.1016/S0165-5728(00)00387-8

δ-opioid receptors inhibit neurogenic intestinal secretion evoked by mast cell degranulation and type I hypersensitivity

Sutthasinee Poonyachoti, David R. Brown

Veterinary and Biomedical Sciences

Research output: Contribution to journal › Article › peer-review

11 Scopus citations

Abstract

Histamine and the mast cell degranulator, compound 48/80 produced elevations in short-circuit current, an electrical measure of active anion secretion, across porcine ileal mucosa sheets mounted in Ussing chambers. Luminally-applied β-lactoglobulin produced similar effects in mucosal sheets from cow's milk-sensitized pigs. Their secretory effects were attenuated by blockers of H₁-histamine receptors, neuronal conduction or epithelial Na⁺/K⁺/Cl^- cotransport. The δ-opioid agonist [D-Pen²,D-Pen⁵]enkephalin suppressed mucosal responses to these substances in a naltrindole-reversible manner. Furthermore, submucosal mast cells and δ-opioid receptor-immunoreactive nerve fibers were observed in close juxtaposition. Intestinal neural pathways linking immediate hypersensitivity to secretory host defense appear to express inhibitory δ-opioid receptors. (C) 2001 Elsevier Science B.V.

Original language	English (US)
Pages (from-to)	89-96
Number of pages	8
Journal	Journal of Neuroimmunology
Volume	112
Issue number	1-2
DOIs	https://doi.org/10.1016/S0165-5728(00)00387-8
State	Published - Jan 1 2001

Bibliographical note

Funding Information:
The authors thank Drs. Mary Pampusch (Department of Animal Science, University of Minnesota), Anjali Kulkarni-Narla and Dennis Foss (Department of Veterinary PathoBiology, University of Minnesota) for excellent technical assistance or advice during this study. We are also grateful for gifts of nor-BNI and anti-δ-opioid receptor antiserum from Drs. Philip Portoghese (Department of Medicinal Chemistry, University of Minnesota) and Robert P. Elde (Department of Neuroscience, University of Minnesota), respectively. This study was funded by NIH grant DA-10200. S.P. was supported by a Royal Thai Government fellowship.

Keywords

Enkephalin
Food allergy
Ileum
Pharmaconeuroimmunology
Pig

Access

10.1016/S0165-5728(00)00387-8

OpenUrl availability

Full text

Cite this

@article{8a01183ef37144c4bfaa821aa78bc5f7,

title = "δ-opioid receptors inhibit neurogenic intestinal secretion evoked by mast cell degranulation and type I hypersensitivity",

abstract = "Histamine and the mast cell degranulator, compound 48/80 produced elevations in short-circuit current, an electrical measure of active anion secretion, across porcine ileal mucosa sheets mounted in Ussing chambers. Luminally-applied β-lactoglobulin produced similar effects in mucosal sheets from cow's milk-sensitized pigs. Their secretory effects were attenuated by blockers of H1-histamine receptors, neuronal conduction or epithelial Na+/K+/Cl- cotransport. The δ-opioid agonist [D-Pen2,D-Pen5]enkephalin suppressed mucosal responses to these substances in a naltrindole-reversible manner. Furthermore, submucosal mast cells and δ-opioid receptor-immunoreactive nerve fibers were observed in close juxtaposition. Intestinal neural pathways linking immediate hypersensitivity to secretory host defense appear to express inhibitory δ-opioid receptors. (C) 2001 Elsevier Science B.V.",

keywords = "Enkephalin, Food allergy, Ileum, Pharmaconeuroimmunology, Pig",

author = "Sutthasinee Poonyachoti and Brown, {David R.}",

note = "Funding Information: The authors thank Drs. Mary Pampusch (Department of Animal Science, University of Minnesota), Anjali Kulkarni-Narla and Dennis Foss (Department of Veterinary PathoBiology, University of Minnesota) for excellent technical assistance or advice during this study. We are also grateful for gifts of nor-BNI and anti-δ-opioid receptor antiserum from Drs. Philip Portoghese (Department of Medicinal Chemistry, University of Minnesota) and Robert P. Elde (Department of Neuroscience, University of Minnesota), respectively. This study was funded by NIH grant DA-10200. S.P. was supported by a Royal Thai Government fellowship. ",

year = "2001",

month = jan,

day = "1",

doi = "10.1016/S0165-5728(00)00387-8",

language = "English (US)",

volume = "112",

pages = "89--96",

journal = "Journal of Neuroimmunology",

issn = "0165-5728",

publisher = "Elsevier",

number = "1-2",

}

TY - JOUR

T1 - δ-opioid receptors inhibit neurogenic intestinal secretion evoked by mast cell degranulation and type I hypersensitivity

AU - Poonyachoti, Sutthasinee

AU - Brown, David R.

N1 - Funding Information: The authors thank Drs. Mary Pampusch (Department of Animal Science, University of Minnesota), Anjali Kulkarni-Narla and Dennis Foss (Department of Veterinary PathoBiology, University of Minnesota) for excellent technical assistance or advice during this study. We are also grateful for gifts of nor-BNI and anti-δ-opioid receptor antiserum from Drs. Philip Portoghese (Department of Medicinal Chemistry, University of Minnesota) and Robert P. Elde (Department of Neuroscience, University of Minnesota), respectively. This study was funded by NIH grant DA-10200. S.P. was supported by a Royal Thai Government fellowship.

PY - 2001/1/1

Y1 - 2001/1/1

N2 - Histamine and the mast cell degranulator, compound 48/80 produced elevations in short-circuit current, an electrical measure of active anion secretion, across porcine ileal mucosa sheets mounted in Ussing chambers. Luminally-applied β-lactoglobulin produced similar effects in mucosal sheets from cow's milk-sensitized pigs. Their secretory effects were attenuated by blockers of H1-histamine receptors, neuronal conduction or epithelial Na+/K+/Cl- cotransport. The δ-opioid agonist [D-Pen2,D-Pen5]enkephalin suppressed mucosal responses to these substances in a naltrindole-reversible manner. Furthermore, submucosal mast cells and δ-opioid receptor-immunoreactive nerve fibers were observed in close juxtaposition. Intestinal neural pathways linking immediate hypersensitivity to secretory host defense appear to express inhibitory δ-opioid receptors. (C) 2001 Elsevier Science B.V.

AB - Histamine and the mast cell degranulator, compound 48/80 produced elevations in short-circuit current, an electrical measure of active anion secretion, across porcine ileal mucosa sheets mounted in Ussing chambers. Luminally-applied β-lactoglobulin produced similar effects in mucosal sheets from cow's milk-sensitized pigs. Their secretory effects were attenuated by blockers of H1-histamine receptors, neuronal conduction or epithelial Na+/K+/Cl- cotransport. The δ-opioid agonist [D-Pen2,D-Pen5]enkephalin suppressed mucosal responses to these substances in a naltrindole-reversible manner. Furthermore, submucosal mast cells and δ-opioid receptor-immunoreactive nerve fibers were observed in close juxtaposition. Intestinal neural pathways linking immediate hypersensitivity to secretory host defense appear to express inhibitory δ-opioid receptors. (C) 2001 Elsevier Science B.V.

KW - Enkephalin

KW - Food allergy

KW - Ileum

KW - Pharmaconeuroimmunology

KW - Pig

UR - http://www.scopus.com/inward/record.url?scp=0035212897&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0035212897&partnerID=8YFLogxK

U2 - 10.1016/S0165-5728(00)00387-8

DO - 10.1016/S0165-5728(00)00387-8

M3 - Article

C2 - 11108937

AN - SCOPUS:0035212897

SN - 0165-5728

VL - 112

SP - 89

EP - 96

JO - Journal of Neuroimmunology

JF - Journal of Neuroimmunology

IS - 1-2

ER -

δ-opioid receptors inhibit neurogenic intestinal secretion evoked by mast cell degranulation and type I hypersensitivity

Abstract

Bibliographical note

Keywords

Access

OpenUrl availability

Other files and links

Fingerprint

Cite this