TY - JOUR
T1 - 3,5,7,3′,4′-Pentamethoxyflavone, a quercetin derivative protects DNA from oxidative challenges
T2 - Potential mechanism of action
AU - Jakhar, Rekha
AU - Paul, Souren
AU - Park, Young Rong
AU - Han, Jaehong
AU - Kang, Sun Chul
PY - 2014/2/5
Y1 - 2014/2/5
N2 - DNA protection is one of the most important strategies in cancer therapy. Since quercetin and its derivatives are found to be potent antioxidant agents, they are able to scavenge radicals significantly. Therefore, we focused on the DNA protection activity of 3,5,7,3′,4′-pentamethoxyflavone (PMF), a quercetin derivative isolated from Kaemperia parviflora. Although, PMF was found to be a very poor antioxidant compound, still it could remarkably protect DNA from oxidative damage. DNA binding assay showed that PMF bound to the minor groove of DNA, which suggests a possible mechanism for its DNA protective effects. Cellular toxicity assay on RAW 264.7 macrophages showed this compound is very safe for therapeutic applications.
AB - DNA protection is one of the most important strategies in cancer therapy. Since quercetin and its derivatives are found to be potent antioxidant agents, they are able to scavenge radicals significantly. Therefore, we focused on the DNA protection activity of 3,5,7,3′,4′-pentamethoxyflavone (PMF), a quercetin derivative isolated from Kaemperia parviflora. Although, PMF was found to be a very poor antioxidant compound, still it could remarkably protect DNA from oxidative damage. DNA binding assay showed that PMF bound to the minor groove of DNA, which suggests a possible mechanism for its DNA protective effects. Cellular toxicity assay on RAW 264.7 macrophages showed this compound is very safe for therapeutic applications.
KW - Antioxidant
KW - Comet assay
KW - DNA binding
KW - DNA damage
KW - ROS
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U2 - 10.1016/j.jphotobiol.2014.01.003
DO - 10.1016/j.jphotobiol.2014.01.003
M3 - Article
C2 - 24514458
AN - SCOPUS:84893701521
SN - 1011-1344
VL - 131
SP - 96
EP - 103
JO - Journal of Photochemistry and Photobiology B: Biology
JF - Journal of Photochemistry and Photobiology B: Biology
ER -
