Abstract
We report Fourier transform infrared spectro-microtomography, a nondestructive three-dimensional imaging approach that reveals the distribution of distinctive chemical compositions throughout an intact biological or materials sample. The method combines mid-infrared absorption contrast with computed tomographic data acquisition and reconstruction to enhance chemical and morphological localization by determining a complete infrared spectrum for every voxel (millions of spectra determined per sample).
| Original language | English (US) |
|---|---|
| Pages (from-to) | 861-864 |
| Number of pages | 4 |
| Journal | Nature Methods |
| Volume | 10 |
| Issue number | 9 |
| DOIs | |
| State | Published - Sep 2013 |
Bibliographical note
Funding Information:Thanks to K. Krueger, M. Fisher and G. Rogers for outstanding machining skills and technical support. We also thank D. Ron for assistance on the spectral extractions. This work is based on research conducted at the IRENI beamline, whose construction and development was supported by the US National Science Foundation by award MRI-0619759. This work was supported by the US National Science Foundation under grant CHE-1112433. The ALS is supported by the Director, Office of Science, Office of Basic Energy Sciences, US Department of Energy under contract no. DE-AC02-05CH11231. The SRC is primarily funded by the University of Wisconsin–Madison, with supplemental support from facility users and the University of Wisconsin–Milwaukee.