Abstract
Induced-pluripotent stem cell (iPSC)-derived hepatocytes (iHeps) is a promising cell source for high-throughput screening and personalized medicine. Current approaches for maturation of iHeps fail to fully recapitulate the cell-extracellular matrix (ECM) and cell-cell interactions that are more akin to liver microenvironment. We leveraged droplet microfluidics to fabricate highly monodisperse, multicellular 3D microtissues that are scalable and provide optimized control over the homotypic and heterotypic interactions between iHeps and supporting non-parenchymal cells (NPCs) of the liver. Utilizing the platform, we systematically investigated the role of heterotypic interactions from fibroblasts and endothelial cells in regulating hepatic functions.
| Original language | English (US) |
|---|---|
| Title of host publication | MicroTAS 2020 - 24th International Conference on Miniaturized Systems for Chemistry and Life Sciences |
| Publisher | Chemical and Biological Microsystems Society |
| Pages | 865-866 |
| Number of pages | 2 |
| ISBN (Electronic) | 9781733419017 |
| State | Published - 2020 |
| Event | 24th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2020 - Virtual, Online Duration: Oct 4 2020 → Oct 9 2020 |
Publication series
| Name | MicroTAS 2020 - 24th International Conference on Miniaturized Systems for Chemistry and Life Sciences |
|---|
Conference
| Conference | 24th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2020 |
|---|---|
| City | Virtual, Online |
| Period | 10/4/20 → 10/9/20 |
Bibliographical note
Publisher Copyright:© 2020 CBMS-0001
Keywords
- Hepatocyte like cells
- IPSC-derived hepatocytes
- Liver development
- Non-parenchymal cells
- Regenerative medicine