A monoclonal anti-peptide antibody mimics adrenocorticotropic hormone activity

B. L. Clarke; K. L. Bost

doi:10.1016/0165-2478(91)90117-S

A monoclonal anti-peptide antibody mimics adrenocorticotropic hormone activity

B. L. Clarke, K. L. Bost

Biomedical Sciences

Research output: Contribution to journal › Article › peer-review

4 Scopus citations

Abstract

A monoclonal antibody that specifically recognizes the adrenocorticotropic receptor (ACTH) on rat adrenal cells was tested for hormonal activity. The antibody behaved as an agonist based on three different biological activities associated with ACTH. An antibody concentration of 16 μg/ml stimulated isolated rat adrenal cells to secrete 800 ng/10⁴ cells of corticosterone with a concomitant 10-fold increase of cAMP to 30 pmol/10⁵ cells. Antibody concentrations above 16 μg/ml inhibited mitotic activity in mouse Y-1 adrenal cells. A radioimmunoassay using an anti-ACTH antibody showed that the monoclonal anti-adrenocorticotropic receptor antibody and ACTH are antigenically related. These findings suggest that the anti-receptor antibody recognizes the ligand binding domain of the ACTH receptor.

Original language	English (US)
Pages (from-to)	175-180
Number of pages	6
Journal	Immunology Letters
Volume	28
Issue number	2
DOIs	https://doi.org/10.1016/0165-2478(91)90117-S
State	Published - May 1991

Bibliographical note

Funding Information:
nett for expert technical assistance. This research was supported by NIDDKD grant DK39299 and PHS training grant AI107150-11.

Keywords

ACTH receptor
Adenylate cyclase
Complementary peptide
Mitosis
Monoclonal antibody
Steroidogenesis

UN SDGs

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title = "A monoclonal anti-peptide antibody mimics adrenocorticotropic hormone activity",

abstract = "A monoclonal antibody that specifically recognizes the adrenocorticotropic receptor (ACTH) on rat adrenal cells was tested for hormonal activity. The antibody behaved as an agonist based on three different biological activities associated with ACTH. An antibody concentration of 16 μg/ml stimulated isolated rat adrenal cells to secrete 800 ng/104 cells of corticosterone with a concomitant 10-fold increase of cAMP to 30 pmol/105 cells. Antibody concentrations above 16 μg/ml inhibited mitotic activity in mouse Y-1 adrenal cells. A radioimmunoassay using an anti-ACTH antibody showed that the monoclonal anti-adrenocorticotropic receptor antibody and ACTH are antigenically related. These findings suggest that the anti-receptor antibody recognizes the ligand binding domain of the ACTH receptor.",

keywords = "ACTH receptor, Adenylate cyclase, Complementary peptide, Mitosis, Monoclonal antibody, Steroidogenesis",

author = "Clarke, {B. L.} and Bost, {K. L.}",

note = "Funding Information: nett for expert technical assistance. This research was supported by NIDDKD grant DK39299 and PHS training grant AI107150-11.",

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doi = "10.1016/0165-2478(91)90117-S",

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AU - Clarke, B. L.

AU - Bost, K. L.

N1 - Funding Information: nett for expert technical assistance. This research was supported by NIDDKD grant DK39299 and PHS training grant AI107150-11.

PY - 1991/5

Y1 - 1991/5

N2 - A monoclonal antibody that specifically recognizes the adrenocorticotropic receptor (ACTH) on rat adrenal cells was tested for hormonal activity. The antibody behaved as an agonist based on three different biological activities associated with ACTH. An antibody concentration of 16 μg/ml stimulated isolated rat adrenal cells to secrete 800 ng/104 cells of corticosterone with a concomitant 10-fold increase of cAMP to 30 pmol/105 cells. Antibody concentrations above 16 μg/ml inhibited mitotic activity in mouse Y-1 adrenal cells. A radioimmunoassay using an anti-ACTH antibody showed that the monoclonal anti-adrenocorticotropic receptor antibody and ACTH are antigenically related. These findings suggest that the anti-receptor antibody recognizes the ligand binding domain of the ACTH receptor.

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KW - ACTH receptor

KW - Adenylate cyclase

KW - Complementary peptide

KW - Mitosis

KW - Monoclonal antibody

KW - Steroidogenesis

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A monoclonal anti-peptide antibody mimics adrenocorticotropic hormone activity

Abstract

Bibliographical note

Keywords

UN SDGs

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