A Multicomponent Animal Virus Isolated from Mosquitoes

Jason T. Ladner; Michael R. Wiley; Brett Beitzel; Albert J. Auguste; Alan P. Dupuis; Michael E. Lindquist; Samuel D. Sibley; Krishna P. Kota; David Fetterer; Gillian Eastwood; David Kimmel; Karla Prieto; Hilda Guzman; Matthew T. Aliota; Daniel Reyes; Ernst E. Brueggemann; Lena St. John; David Hyeroba; Michael Lauck; Thomas C. Friedrich; David H. O'Connor; Marie C. Gestole; Lisa H. Cazares; Vsevolod L. Popov; Fanny Castro-Llanos; Tadeusz J. Kochel; Tara Kenny; Bailey White; Michael D. Ward; Jose R. Loaiza; Tony L. Goldberg; Scott C. Weaver; Laura D. Kramer; Robert B. Tesh; Gustavo Palacios

doi:10.1016/j.chom.2016.07.011

A Multicomponent Animal Virus Isolated from Mosquitoes

Jason T. Ladner, Michael R. Wiley, Brett Beitzel, Albert J. Auguste, Alan P. Dupuis, Michael E. Lindquist, Samuel D. Sibley, Krishna P. Kota, David Fetterer, Gillian Eastwood, David Kimmel, Karla Prieto, Hilda Guzman, Matthew T. Aliota, Daniel Reyes, Ernst E. Brueggemann, Lena St. John, David Hyeroba, Michael Lauck, Thomas C. FriedrichDavid H. O'Connor, Marie C. Gestole, Lisa H. Cazares, Vsevolod L. Popov, Fanny Castro-Llanos, Tadeusz J. Kochel, Tara Kenny, Bailey White, Michael D. Ward, Jose R. Loaiza, Tony L. Goldberg, Scott C. Weaver, Laura D. Kramer, Robert B. Tesh, Gustavo Palacios

Veterinary and Biomedical Sciences

Research output: Contribution to journal › Article › peer-review

108 Scopus citations

Abstract

RNA viruses exhibit a variety of genome organization strategies, including multicomponent genomes in which each segment is packaged separately. Although multicomponent genomes are common among viruses infecting plants and fungi, their prevalence among those infecting animals remains unclear. We characterize a multicomponent RNA virus isolated from mosquitoes, designated Guaico Culex virus (GCXV). GCXV belongs to a diverse clade of segmented viruses (Jingmenvirus) related to the prototypically unsegmented Flaviviridae. The GCXV genome comprises five segments, each of which appears to be separately packaged. The smallest segment is not required for replication, and its presence is variable in natural infections. We also describe a variant of Jingmen tick virus, another Jingmenvirus, sequenced from a Ugandan red colobus monkey, thus expanding the host range of this segmented and likely multicomponent virus group. Collectively, this study provides evidence for the existence of multicomponent animal viruses and their potential relevance for animal and human health.

Original language	English (US)
Pages (from-to)	357-367
Number of pages	11
Journal	Cell Host and Microbe
Volume	20
Issue number	3
DOIs	https://doi.org/10.1016/j.chom.2016.07.011
State	Published - Sep 14 2016

Bibliographical note

Funding Information:
Work at U.S. Army Medical Research Institute of Infectious Diseases was funded by the Defense Threat Reduction Agency, project 1881290. Work at UTMB was supported by NIH contract HHSN272201000040I/HHSN27200004/D04 to R.B.T. A.J.A. was supported by the James W. McLaughlin Endowment fund. Mosquito collection in Panama was funded by Smithsonian Tropical Research Institute – Environmental Protection Agency grant DW33-92296801-0 to Montira J. Pongsiri. Work in Panama was supported by a Robert E. Shope fellowship awarded to G.E.

Publisher Copyright:
© 2016 Elsevier Inc.

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Ladner, J. T., Wiley, M. R., Beitzel, B., Auguste, A. J., Dupuis, A. P., Lindquist, M. E., Sibley, S. D., Kota, K. P., Fetterer, D., Eastwood, G., Kimmel, D., Prieto, K., Guzman, H., Aliota, M. T., Reyes, D., Brueggemann, E. E., St. John, L., Hyeroba, D., Lauck, M., ... Palacios, G. (2016). A Multicomponent Animal Virus Isolated from Mosquitoes. Cell Host and Microbe, 20(3), 357-367. https://doi.org/10.1016/j.chom.2016.07.011

Ladner, JT, Wiley, MR, Beitzel, B, Auguste, AJ, Dupuis, AP, Lindquist, ME, Sibley, SD, Kota, KP, Fetterer, D, Eastwood, G, Kimmel, D, Prieto, K, Guzman, H, Aliota, MT, Reyes, D, Brueggemann, EE, St. John, L, Hyeroba, D, Lauck, M, Friedrich, TC, O'Connor, DH, Gestole, MC, Cazares, LH, Popov, VL, Castro-Llanos, F, Kochel, TJ, Kenny, T, White, B, Ward, MD, Loaiza, JR, Goldberg, TL, Weaver, SC, Kramer, LD, Tesh, RB & Palacios, G 2016, 'A Multicomponent Animal Virus Isolated from Mosquitoes', Cell Host and Microbe, vol. 20, no. 3, pp. 357-367. https://doi.org/10.1016/j.chom.2016.07.011

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abstract = "RNA viruses exhibit a variety of genome organization strategies, including multicomponent genomes in which each segment is packaged separately. Although multicomponent genomes are common among viruses infecting plants and fungi, their prevalence among those infecting animals remains unclear. We characterize a multicomponent RNA virus isolated from mosquitoes, designated Guaico Culex virus (GCXV). GCXV belongs to a diverse clade of segmented viruses (Jingmenvirus) related to the prototypically unsegmented Flaviviridae. The GCXV genome comprises five segments, each of which appears to be separately packaged. The smallest segment is not required for replication, and its presence is variable in natural infections. We also describe a variant of Jingmen tick virus, another Jingmenvirus, sequenced from a Ugandan red colobus monkey, thus expanding the host range of this segmented and likely multicomponent virus group. Collectively, this study provides evidence for the existence of multicomponent animal viruses and their potential relevance for animal and human health.",

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note = "Funding Information: Work at U.S. Army Medical Research Institute of Infectious Diseases was funded by the Defense Threat Reduction Agency, project 1881290. Work at UTMB was supported by NIH contract HHSN272201000040I/HHSN27200004/D04 to R.B.T. A.J.A. was supported by the James W. McLaughlin Endowment fund. Mosquito collection in Panama was funded by Smithsonian Tropical Research Institute – Environmental Protection Agency grant DW33-92296801-0 to Montira J. Pongsiri. Work in Panama was supported by a Robert E. Shope fellowship awarded to G.E. Publisher Copyright: {\textcopyright} 2016 Elsevier Inc.",

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AU - Sibley, Samuel D.

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AU - Kimmel, David

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AU - Guzman, Hilda

AU - Aliota, Matthew T.

AU - Reyes, Daniel

AU - Brueggemann, Ernst E.

AU - St. John, Lena

AU - Hyeroba, David

AU - Lauck, Michael

AU - Friedrich, Thomas C.

AU - O'Connor, David H.

AU - Gestole, Marie C.

AU - Cazares, Lisa H.

AU - Popov, Vsevolod L.

AU - Castro-Llanos, Fanny

AU - Kochel, Tadeusz J.

AU - Kenny, Tara

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AU - Loaiza, Jose R.

AU - Goldberg, Tony L.

AU - Weaver, Scott C.

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AU - Tesh, Robert B.

AU - Palacios, Gustavo

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A Multicomponent Animal Virus Isolated from Mosquitoes

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