A nodule-specific protein secretory pathway required for nitrogen-fixing symbiosis

Dong Wang; Joel Griffitts; Colby Starker; Elena Fedorova; Erik Limpens; Sergey Ivanov; Ton Bisseling; Sharon Long

doi:10.1126/science.1184096

A nodule-specific protein secretory pathway required for nitrogen-fixing symbiosis

Dong Wang, Joel Griffitts, Colby Starker, Elena Fedorova, Erik Limpens, Sergey Ivanov, Ton Bisseling, Sharon Long

Genetics, Cell Biology and Development (CBS)

Research output: Contribution to journal › Article › peer-review

224 Scopus citations

Abstract

The nitrogen-fixing symbiosis between Sinorhizobium meliloti and its leguminous host plant Medicago truncatula occurs in a specialized root organ called the nodule. Bacteria that are released into plant cells are surrounded by a unique plant membrane compartment termed a symbiosome. We found that in the symbiosis-defective dnf1 mutant of M. truncatula, bacteroid and symbiosome development are blocked. We identified the DNF1 gene as encoding a subunit of a signal peptidase complex that is highly expressed in nodules. By analyzing data from whole-genome expression analysis, we propose that correct symbiosome development in M. truncatula requires the orderly secretion of protein constituents through coordinated up-regulation of a nodule-specific pathway exemplified by DNF1.

Original language	English (US)
Pages (from-to)	1126-1129
Number of pages	4
Journal	Science
Volume	327
Issue number	5969
DOIs	https://doi.org/10.1126/science.1184096
State	Published - Feb 26 2010

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abstract = "The nitrogen-fixing symbiosis between Sinorhizobium meliloti and its leguminous host plant Medicago truncatula occurs in a specialized root organ called the nodule. Bacteria that are released into plant cells are surrounded by a unique plant membrane compartment termed a symbiosome. We found that in the symbiosis-defective dnf1 mutant of M. truncatula, bacteroid and symbiosome development are blocked. We identified the DNF1 gene as encoding a subunit of a signal peptidase complex that is highly expressed in nodules. By analyzing data from whole-genome expression analysis, we propose that correct symbiosome development in M. truncatula requires the orderly secretion of protein constituents through coordinated up-regulation of a nodule-specific pathway exemplified by DNF1.",

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AU - Wang, Dong

AU - Griffitts, Joel

AU - Starker, Colby

AU - Fedorova, Elena

AU - Limpens, Erik

AU - Ivanov, Sergey

AU - Bisseling, Ton

AU - Long, Sharon

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A nodule-specific protein secretory pathway required for nitrogen-fixing symbiosis

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