Abstract
The RB gene from wild potato Solanum bulbocastanum imparts broad-spectrum late blight resistance to cultivated potato. To explore marker associations and haplotype frequencies near RB, we developed, optimized, validated, and employed a set of markers specific to the haplotype associated with the RB resistance allele. Our markers, developed using a mismatch amplification mutation assay (MAMA)-PCR approach, have single nucleotide polymorphism-level sensitivity. MAMA markers were validated via linkage mapping and applied to a collection of 60 S. bulbocastanum genotypes representing the geographic distribution of the species. Considerable marker disassociation within a 100 kb region encompassing RB was revealed. Physical distance between markers was not an effective predictor of marker disassociation. Applied to a collection of four S. bulbocastanum genotypes of known RB allelic status, a set of six MAMA markers accurately predicted the presence of the RB allele. Implications for marker aided genotype selection and association mapping in wild potato species are explored.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 269-282 |
| Number of pages | 14 |
| Journal | American Journal of Potato Research |
| Volume | 88 |
| Issue number | 3 |
| DOIs | |
| State | Published - Jun 2011 |
Bibliographical note
Funding Information:Acknowledgements This project was partially funded by the NSF Potato Genome Project (DBI0218166). We thank Dr. John Helgeson, USDA-ARS and University of Wisconsin for providing S. bulbocas-tanum genotypes PT29 and SB22 and Dr. Chuck Brown, USDA-ARS for providing the S. bulbocastanum mapping population. Computer support from the University of Minnesota Supercomputing Institute is gratefully acknowledged. All experiments herein reported comply with current U.S. laws.
Keywords
- Late blight resistance
- MAMA-PCR
- Potato
- RB gene
- Solanum bulbocastanum
