Abstract
HIV-1 envelope glycoproteins (Envs) bind to CD4 receptor and CCR5/CXCR4 coreceptor and mediate viral entry (Feng et al., 1996; Herschhorn et al., 2016, 2017; Kwong et al., 1998). HIV-1 Envs are the sole target of neutralizing antibodies and a main focus of vaccine development (Flemming et al., 2018). Here, we provide a step-by-step protocol to measure Env sensitivity to ligands, cold, and small molecules, as well as to study viral infectivity and to dissect parameters affecting HIV-1 Env function. For complete details on the use and execution of this protocol, please refer to Harris et al. (2020).
| Original language | English (US) |
|---|---|
| Article number | 100133 |
| Journal | STAR Protocols |
| Volume | 1 |
| Issue number | 3 |
| DOIs | |
| State | Published - Dec 18 2020 |
Bibliographical note
Funding Information:We thank Eva Perez-Greene for critical reading of the manuscript. A.H. is the recipient of an amfAR Mathilde Krim Fellowship in Basic Biomedical Research (108501-53-RKNT) and a phase II amfAR research grant (109285-58-RKVA) for independent investigators. This work was supported by internal funds of the Department of Medicine at the University of Minnesota and by NIH/ NIDA grant 1DP2DA049279-01 (to A.H.). The content is solely the responsibility of the authors and does not necessarily represent the official views of the NIH.
Publisher Copyright:
© 2020 The Author(s)
