Abstract
A recombinant construct ptMTa-scGH containing the silver carp growth hormone gene and a promoter-enhancer area of the rainbow trout metal lothionein A gene was microinjected in the cytoplasm of the fertilized carp eggs. It was shown that ptMTa-scGH in the ring form as a part of Bluescript plasmid vector induced specific developmental defects and death in the embryos. No such pathogenic effect was found when a linearized fragment tMTa-scGH was used. Sequences of the introduced genes were found in transgenic carps: fingerlings, one-and two-year old individuals. The results of testing of the studied construct in vivo suggest the possible use of linearized fragment tMTa-scGH in experiments aimed at the production of genetically modified carps.
Original language | English (US) |
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Pages (from-to) | 286-287 |
Number of pages | 2 |
Journal | Ontogenez |
Volume | 27 |
Issue number | 5 |
State | Published - 1996 |
Keywords
- Carp
- Recombinant DNA
- Somatotropin gene
- Transgene