TY - JOUR
T1 - A T cell receptor Vα region selectively expressed in CD4+ cells
AU - Jameson, Stephen C.
AU - Kaye, Jonathan
AU - Gascoigne, Nicholas R J
PY - 1990
Y1 - 1990
N2 - The peripheral TCR Vβ repertoire is strongly influenced by the processes of negative selection (deletion) and positive selection in the thymus. In order to investigate whether such selection events influence the Vα repertoire, we have produced an anti-Vα11 mAb. This antibody was made by immunization with a chimeric TCR:Ig protein containing Vα11 in place of the V(H) of an IgG2a,λ Ig. This scheme optimizes the specificity of immunization and facilitates the screening procedure. The anbtibody recognizes a panel of Vα11-expressing T cell clones. Analysis of mouse strains indicates that the antibody recognizes Vα11 only in mice of the C57 background. The expression of the epitope on peripheral T cells is strongly biased to the CD4+ subset, suggesting positive selection of Vα11 on class II MHC molecules. In some strain comparisons, the percentage of Vα11-expressing T cells in the CD4+ subset was elevated in I-E+ relative to I-E- strains. These data suggest that Vα11 can differentially influence the selection of T cells into the CD4+/CD8+ subsets.
AB - The peripheral TCR Vβ repertoire is strongly influenced by the processes of negative selection (deletion) and positive selection in the thymus. In order to investigate whether such selection events influence the Vα repertoire, we have produced an anti-Vα11 mAb. This antibody was made by immunization with a chimeric TCR:Ig protein containing Vα11 in place of the V(H) of an IgG2a,λ Ig. This scheme optimizes the specificity of immunization and facilitates the screening procedure. The anbtibody recognizes a panel of Vα11-expressing T cell clones. Analysis of mouse strains indicates that the antibody recognizes Vα11 only in mice of the C57 background. The expression of the epitope on peripheral T cells is strongly biased to the CD4+ subset, suggesting positive selection of Vα11 on class II MHC molecules. In some strain comparisons, the percentage of Vα11-expressing T cells in the CD4+ subset was elevated in I-E+ relative to I-E- strains. These data suggest that Vα11 can differentially influence the selection of T cells into the CD4+/CD8+ subsets.
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M3 - Article
C2 - 1696594
AN - SCOPUS:0025161355
SN - 0022-1767
VL - 145
SP - 1324
EP - 1331
JO - Journal of Immunology
JF - Journal of Immunology
IS - 5
ER -