AAV-mediated gene delivery to the spinal cord by intrathecal injection

Cristina D. Peterson; Alexander G.J. Skorput; Kelley F Kitto; George L Wilcox; Lucy Vulchanova; Carolyn A Fairbanks

doi:10.1007/978-1-4939-9139-6_11

AAV-mediated gene delivery to the spinal cord by intrathecal injection

Cristina D. Peterson, Alexander G.J. Skorput, Kelley F Kitto, George L Wilcox, Lucy Vulchanova, Carolyn A Fairbanks

Research output: Chapter in Book/Report/Conference proceeding › Chapter

3 Scopus citations

Abstract

Gene therapy targeting the spinal cord is an important tool for analyzing mechanisms of nervous system diseases and the development of gene therapies. Analogous to a lumbar puncture in humans, the rodent spinal cord can be accessed through an efficient, noninvasive injection. Here we describe a method for AAV-mediated gene transfer to cells of the spinal cord by intrathecal injection of small quantities of AAV vector.

Original language	English (US)
Title of host publication	Methods in Molecular Biology
Publisher	Humana Press Inc.
Pages	199-207
Number of pages	9
DOIs	https://doi.org/10.1007/978-1-4939-9139-6_11
State	Published - 2019

Publication series

Name	Methods in Molecular Biology
Volume	1950
ISSN (Print)	1064-3745

Bibliographical note

Publisher Copyright:
© Springer Science+Business Media, LLC, part of Springer Nature 2019.

Keywords

Adeno-associated
Dorsal root ganglion
Spinal cord
Viral vector

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10.1007/978-1-4939-9139-6_11

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abstract = "Gene therapy targeting the spinal cord is an important tool for analyzing mechanisms of nervous system diseases and the development of gene therapies. Analogous to a lumbar puncture in humans, the rodent spinal cord can be accessed through an efficient, noninvasive injection. Here we describe a method for AAV-mediated gene transfer to cells of the spinal cord by intrathecal injection of small quantities of AAV vector.",

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AU - Skorput, Alexander G.J.

AU - Kitto, Kelley F

AU - Wilcox, George L

AU - Vulchanova, Lucy

AU - Fairbanks, Carolyn A

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AB - Gene therapy targeting the spinal cord is an important tool for analyzing mechanisms of nervous system diseases and the development of gene therapies. Analogous to a lumbar puncture in humans, the rodent spinal cord can be accessed through an efficient, noninvasive injection. Here we describe a method for AAV-mediated gene transfer to cells of the spinal cord by intrathecal injection of small quantities of AAV vector.

KW - Adeno-associated

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KW - Spinal cord

KW - Viral vector

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ER -

AAV-mediated gene delivery to the spinal cord by intrathecal injection

Abstract

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