Aegilops sharonensis genome-assisted identification of stem rust resistance gene Sr62

Guotai Yu; Oadi Matny; Nicolas Champouret; Burkhard Steuernagel; Matthew J. Moscou; Inmaculada Hernández-Pinzón; Phon Green; Sadiye Hayta; Mark Smedley; Wendy Harwood; Ngonidzashe Kangara; Yajuan Yue; Catherine Gardener; Mark J. Banfield; Pablo D. Olivera; Cole Welchin; Jamie Simmons; Eitan Millet; Anna Minz-Dub; Moshe Ronen; Raz Avni; Amir Sharon; Mehran Patpour; Annemarie F. Justesen; Murukarthick Jayakodi; Axel Himmelbach; Nils Stein; Shuangye Wu; Jesse Poland; Jennifer Ens; Curtis Pozniak; Miroslava Karafiátová; István Molnár; Jaroslav Doležel; Eric R. Ward; T. Lynne Reuber; Jonathan D.G. Jones; Martin Mascher; Brian J. Steffenson; Brande B.H. Wulff

doi:10.1038/s41467-022-29132-8

Aegilops sharonensis genome-assisted identification of stem rust resistance gene Sr62

Guotai Yu, Oadi Matny, Nicolas Champouret, Burkhard Steuernagel, Matthew J. Moscou, Inmaculada Hernández-Pinzón, Phon Green, Sadiye Hayta, Mark Smedley, Wendy Harwood, Ngonidzashe Kangara, Yajuan Yue, Catherine Gardener, Mark J. Banfield, Pablo D. Olivera, Cole Welchin, Jamie Simmons, Eitan Millet, Anna Minz-Dub, Moshe RonenRaz Avni, Amir Sharon, Mehran Patpour, Annemarie F. Justesen, Murukarthick Jayakodi, Axel Himmelbach, Nils Stein, Shuangye Wu, Jesse Poland, Jennifer Ens, Curtis Pozniak, Miroslava Karafiátová, István Molnár, Jaroslav Doležel, Eric R. Ward, T. Lynne Reuber, Jonathan D.G. Jones, Martin Mascher, Brian J. Steffenson, Brande B.H. Wulff

Plant Pathology

Research output: Contribution to journal › Article › peer-review

41 Scopus citations

Abstract

The wild relatives and progenitors of wheat have been widely used as sources of disease resistance (R) genes. Molecular identification and characterization of these R genes facilitates their manipulation and tracking in breeding programmes. Here, we develop a reference-quality genome assembly of the wild diploid wheat relative Aegilops sharonensis and use positional mapping, mutagenesis, RNA-Seq and transgenesis to identify the stem rust resistance gene Sr62, which has also been transferred to common wheat. This gene encodes a tandem kinase, homologues of which exist across multiple taxa in the plant kingdom. Stable Sr62 transgenic wheat lines show high levels of resistance against diverse isolates of the stem rust pathogen, highlighting the utility of Sr62 for deployment as part of a polygenic stack to maximize the durability of stem rust resistance.

Original language	English (US)
Article number	1607
Journal	Nature communications
Volume	13
Issue number	1
DOIs	https://doi.org/10.1038/s41467-022-29132-8
State	Published - Dec 2022

Bibliographical note

Publisher Copyright:
© 2022, The Author(s).

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

Access

10.1038/s41467-022-29132-8

OpenUrl availability

Full text

Cite this

Yu, G., Matny, O., Champouret, N., Steuernagel, B., Moscou, M. J., Hernández-Pinzón, I., Green, P., Hayta, S., Smedley, M., Harwood, W., Kangara, N., Yue, Y., Gardener, C., Banfield, M. J., Olivera, P. D., Welchin, C., Simmons, J., Millet, E., Minz-Dub, A., ... Wulff, B. B. H. (2022). Aegilops sharonensis genome-assisted identification of stem rust resistance gene Sr62. Nature communications, 13(1), Article 1607. https://doi.org/10.1038/s41467-022-29132-8

Yu, G, Matny, O, Champouret, N, Steuernagel, B, Moscou, MJ, Hernández-Pinzón, I, Green, P, Hayta, S, Smedley, M, Harwood, W, Kangara, N, Yue, Y, Gardener, C, Banfield, MJ, Olivera, PD, Welchin, C, Simmons, J, Millet, E, Minz-Dub, A, Ronen, M, Avni, R, Sharon, A, Patpour, M, Justesen, AF, Jayakodi, M, Himmelbach, A, Stein, N, Wu, S, Poland, J, Ens, J, Pozniak, C, Karafiátová, M, Molnár, I, Doležel, J, Ward, ER, Reuber, TL, Jones, JDG, Mascher, M, Steffenson, BJ & Wulff, BBH 2022, 'Aegilops sharonensis genome-assisted identification of stem rust resistance gene Sr62', Nature communications, vol. 13, no. 1, 1607. https://doi.org/10.1038/s41467-022-29132-8

@article{7d90e43dadaa4e9a8df0d3da2975a89a,

title = "Aegilops sharonensis genome-assisted identification of stem rust resistance gene Sr62",

abstract = "The wild relatives and progenitors of wheat have been widely used as sources of disease resistance (R) genes. Molecular identification and characterization of these R genes facilitates their manipulation and tracking in breeding programmes. Here, we develop a reference-quality genome assembly of the wild diploid wheat relative Aegilops sharonensis and use positional mapping, mutagenesis, RNA-Seq and transgenesis to identify the stem rust resistance gene Sr62, which has also been transferred to common wheat. This gene encodes a tandem kinase, homologues of which exist across multiple taxa in the plant kingdom. Stable Sr62 transgenic wheat lines show high levels of resistance against diverse isolates of the stem rust pathogen, highlighting the utility of Sr62 for deployment as part of a polygenic stack to maximize the durability of stem rust resistance.",

author = "Guotai Yu and Oadi Matny and Nicolas Champouret and Burkhard Steuernagel and Moscou, {Matthew J.} and Inmaculada Hern{\'a}ndez-Pinz{\'o}n and Phon Green and Sadiye Hayta and Mark Smedley and Wendy Harwood and Ngonidzashe Kangara and Yajuan Yue and Catherine Gardener and Banfield, {Mark J.} and Olivera, {Pablo D.} and Cole Welchin and Jamie Simmons and Eitan Millet and Anna Minz-Dub and Moshe Ronen and Raz Avni and Amir Sharon and Mehran Patpour and Justesen, {Annemarie F.} and Murukarthick Jayakodi and Axel Himmelbach and Nils Stein and Shuangye Wu and Jesse Poland and Jennifer Ens and Curtis Pozniak and Miroslava Karafi{\'a}tov{\'a} and Istv{\'a}n Moln{\'a}r and Jaroslav Dole{\v z}el and Ward, {Eric R.} and Reuber, {T. Lynne} and Jones, {Jonathan D.G.} and Martin Mascher and Steffenson, {Brian J.} and Wulff, {Brande B.H.}",

note = "Publisher Copyright: {\textcopyright} 2022, The Author(s).",

year = "2022",

month = dec,

doi = "10.1038/s41467-022-29132-8",

language = "English (US)",

volume = "13",

journal = "Nature communications",

issn = "2041-1723",

publisher = "Nature Publishing Group",

number = "1",

}

TY - JOUR

T1 - Aegilops sharonensis genome-assisted identification of stem rust resistance gene Sr62

AU - Yu, Guotai

AU - Matny, Oadi

AU - Champouret, Nicolas

AU - Steuernagel, Burkhard

AU - Moscou, Matthew J.

AU - Hernández-Pinzón, Inmaculada

AU - Green, Phon

AU - Hayta, Sadiye

AU - Smedley, Mark

AU - Harwood, Wendy

AU - Kangara, Ngonidzashe

AU - Yue, Yajuan

AU - Gardener, Catherine

AU - Banfield, Mark J.

AU - Olivera, Pablo D.

AU - Welchin, Cole

AU - Simmons, Jamie

AU - Millet, Eitan

AU - Minz-Dub, Anna

AU - Ronen, Moshe

AU - Avni, Raz

AU - Sharon, Amir

AU - Patpour, Mehran

AU - Justesen, Annemarie F.

AU - Jayakodi, Murukarthick

AU - Himmelbach, Axel

AU - Stein, Nils

AU - Wu, Shuangye

AU - Poland, Jesse

AU - Ens, Jennifer

AU - Pozniak, Curtis

AU - Karafiátová, Miroslava

AU - Molnár, István

AU - Doležel, Jaroslav

AU - Ward, Eric R.

AU - Reuber, T. Lynne

AU - Jones, Jonathan D.G.

AU - Mascher, Martin

AU - Steffenson, Brian J.

AU - Wulff, Brande B.H.

PY - 2022/12

Y1 - 2022/12

N2 - The wild relatives and progenitors of wheat have been widely used as sources of disease resistance (R) genes. Molecular identification and characterization of these R genes facilitates their manipulation and tracking in breeding programmes. Here, we develop a reference-quality genome assembly of the wild diploid wheat relative Aegilops sharonensis and use positional mapping, mutagenesis, RNA-Seq and transgenesis to identify the stem rust resistance gene Sr62, which has also been transferred to common wheat. This gene encodes a tandem kinase, homologues of which exist across multiple taxa in the plant kingdom. Stable Sr62 transgenic wheat lines show high levels of resistance against diverse isolates of the stem rust pathogen, highlighting the utility of Sr62 for deployment as part of a polygenic stack to maximize the durability of stem rust resistance.

AB - The wild relatives and progenitors of wheat have been widely used as sources of disease resistance (R) genes. Molecular identification and characterization of these R genes facilitates their manipulation and tracking in breeding programmes. Here, we develop a reference-quality genome assembly of the wild diploid wheat relative Aegilops sharonensis and use positional mapping, mutagenesis, RNA-Seq and transgenesis to identify the stem rust resistance gene Sr62, which has also been transferred to common wheat. This gene encodes a tandem kinase, homologues of which exist across multiple taxa in the plant kingdom. Stable Sr62 transgenic wheat lines show high levels of resistance against diverse isolates of the stem rust pathogen, highlighting the utility of Sr62 for deployment as part of a polygenic stack to maximize the durability of stem rust resistance.

UR - http://www.scopus.com/inward/record.url?scp=85127282541&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85127282541&partnerID=8YFLogxK

U2 - 10.1038/s41467-022-29132-8

DO - 10.1038/s41467-022-29132-8

M3 - Article

C2 - 35338132

AN - SCOPUS:85127282541

SN - 2041-1723

VL - 13

JO - Nature communications

JF - Nature communications

IS - 1

M1 - 1607

ER -

Aegilops sharonensis genome-assisted identification of stem rust resistance gene Sr62

Abstract

Bibliographical note

UN SDGs

Access

OpenUrl availability

Other files and links

Fingerprint

Cite this