TY - JOUR
T1 - Alternaric acid stimulates phosphorylation of His-tagged RiCDPK2, a calcium-dependent protein kinase in potato plants.
AU - Hassan, A.
AU - Okuta, T.
AU - Kato, M.
AU - Hatsugai, N.
AU - Sano, Y.
AU - Ishimori, T.
AU - Okazaki, K.
AU - Doullah, M. A.
AU - Shah, M. M.
PY - 2012
Y1 - 2012
N2 - Calcium-dependent protein kinases (CDPK) are an essential component of plant defense mechanisms against pathogens. We investigated the effect of alternaric acid, a host-specific toxin produced by the plant fungal pathogen Alternaria solani (Pleosporaceae), on a putative plasma membrane and cytosolic kinase RiCDPK2 of potato (Solanum tuberosum) and on hypersensitive cell death of host potato cells. Alternaric acid, in the presence of Ca2+ and Mg2+, stimulated in vitro phosphorylation of His-tagged RiCDPK2, a Ca2+-dependent protein kinase found in potato plants. We concluded that Ca2+ and Mg2+ play an important role in the interaction between alternaric acid and RiCDPK2. Based on our observations, alternaric acid regulates RiCDPK2 kinase during the infection process in an interaction between host and A. solani, leading to the inhibition of hypersensitive cell death in the host. We suggest that alternaric acid is a primary determinant by which A. solani stimulates CDPK activity in the host, suppressing hypersensitive cell death.
AB - Calcium-dependent protein kinases (CDPK) are an essential component of plant defense mechanisms against pathogens. We investigated the effect of alternaric acid, a host-specific toxin produced by the plant fungal pathogen Alternaria solani (Pleosporaceae), on a putative plasma membrane and cytosolic kinase RiCDPK2 of potato (Solanum tuberosum) and on hypersensitive cell death of host potato cells. Alternaric acid, in the presence of Ca2+ and Mg2+, stimulated in vitro phosphorylation of His-tagged RiCDPK2, a Ca2+-dependent protein kinase found in potato plants. We concluded that Ca2+ and Mg2+ play an important role in the interaction between alternaric acid and RiCDPK2. Based on our observations, alternaric acid regulates RiCDPK2 kinase during the infection process in an interaction between host and A. solani, leading to the inhibition of hypersensitive cell death in the host. We suggest that alternaric acid is a primary determinant by which A. solani stimulates CDPK activity in the host, suppressing hypersensitive cell death.
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U2 - 10.4238/2012.May.10.1
DO - 10.4238/2012.May.10.1
M3 - Article
C2 - 22614453
AN - SCOPUS:84872023937
SN - 1676-5680
VL - 11
SP - 2381
EP - 2389
JO - Genetics and molecular research : GMR
JF - Genetics and molecular research : GMR
IS - 3
ER -