Amyloid in the pancreatic islets of the cougar (Felis concolor) is derived from islet amyloid polypeptide (IAPP)

Kenneth H. Johnson; Christer Wernsted; Timothy D. O'Brien; Per Westermark

doi:10.1016/0305-0491(91)90316-6

Amyloid in the pancreatic islets of the cougar (Felis concolor) is derived from islet amyloid polypeptide (IAPP)

Kenneth H. Johnson, Christer Wernsted, Timothy D. O'Brien, Per Westermark

Veterinary Population Medicine

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Abstract

1. 1. Islet amyloid isolated from the pancreas of a 20-year-old cougar (Felis concolor) was dissolved and purified by gel permeation and reversed phase HPLC mino acid sequence analysis. 2. 2. N-Terminal amino acid sequence analysis of the purified protein revealed a primary structure (positions 1-28) identical to islet amyloid polypeptide (IAPP) from domesticated cats. 3. 3. IAPP from the cougar, like IAPP from the human and domesticated cat, incorporates an inherently amyloidogenic AILS sequence at positions 25-28.

Original language	English (US)
Pages (from-to)	115-119
Number of pages	5
Journal	Comparative Biochemistry and Physiology -- Part B: Biochemistry and
Volume	98
Issue number	1
DOIs	https://doi.org/10.1016/0305-0491(91)90316-6
State	Published - 1991

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Funding Information:
Acknowledgements--This work was supportedb y Grant ROI DK36734o f the National Institoutfe D iabeteas nd Digestivea nd Kidney Disease, the SweMdeisdhi calR e-search Council (Project5 N9o4.1 )t,h e Nordic Insulin Fund, and A. Louis-Hansen's Memorial Fund. The authors wish to thankK athyJ ordan,K athyB urg, and Christer Bergman for their technicaasls istance.

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title = "Amyloid in the pancreatic islets of the cougar (Felis concolor) is derived from islet amyloid polypeptide (IAPP)",

abstract = "1. 1. Islet amyloid isolated from the pancreas of a 20-year-old cougar (Felis concolor) was dissolved and purified by gel permeation and reversed phase HPLC mino acid sequence analysis. 2. 2. N-Terminal amino acid sequence analysis of the purified protein revealed a primary structure (positions 1-28) identical to islet amyloid polypeptide (IAPP) from domesticated cats. 3. 3. IAPP from the cougar, like IAPP from the human and domesticated cat, incorporates an inherently amyloidogenic AILS sequence at positions 25-28.",

author = "Johnson, {Kenneth H.} and Christer Wernsted and O'Brien, {Timothy D.} and Per Westermark",

note = "Funding Information: Acknowledgements--This work was supportedb y Grant ROI DK36734o f the National Institoutfe D iabeteas nd Digestivea nd Kidney Disease, the SweMdeisdhi calR e-search Council (Project5 N9o4.1 )t,h e Nordic Insulin Fund, and A. Louis-Hansen's Memorial Fund. The authors wish to thankK athyJ ordan,K athyB urg, and Christer Bergman for their technicaasls istance.",

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T1 - Amyloid in the pancreatic islets of the cougar (Felis concolor) is derived from islet amyloid polypeptide (IAPP)

AU - Johnson, Kenneth H.

AU - Wernsted, Christer

AU - O'Brien, Timothy D.

AU - Westermark, Per

N1 - Funding Information: Acknowledgements--This work was supportedb y Grant ROI DK36734o f the National Institoutfe D iabeteas nd Digestivea nd Kidney Disease, the SweMdeisdhi calR e-search Council (Project5 N9o4.1 )t,h e Nordic Insulin Fund, and A. Louis-Hansen's Memorial Fund. The authors wish to thankK athyJ ordan,K athyB urg, and Christer Bergman for their technicaasls istance.

PY - 1991

Y1 - 1991

N2 - 1. 1. Islet amyloid isolated from the pancreas of a 20-year-old cougar (Felis concolor) was dissolved and purified by gel permeation and reversed phase HPLC mino acid sequence analysis. 2. 2. N-Terminal amino acid sequence analysis of the purified protein revealed a primary structure (positions 1-28) identical to islet amyloid polypeptide (IAPP) from domesticated cats. 3. 3. IAPP from the cougar, like IAPP from the human and domesticated cat, incorporates an inherently amyloidogenic AILS sequence at positions 25-28.

AB - 1. 1. Islet amyloid isolated from the pancreas of a 20-year-old cougar (Felis concolor) was dissolved and purified by gel permeation and reversed phase HPLC mino acid sequence analysis. 2. 2. N-Terminal amino acid sequence analysis of the purified protein revealed a primary structure (positions 1-28) identical to islet amyloid polypeptide (IAPP) from domesticated cats. 3. 3. IAPP from the cougar, like IAPP from the human and domesticated cat, incorporates an inherently amyloidogenic AILS sequence at positions 25-28.

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Amyloid in the pancreatic islets of the cougar (Felis concolor) is derived from islet amyloid polypeptide (IAPP)

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