An efficient protocol for preparing linear β-manno-oligosaccharides

Liangnan Cui; Xiang Wang; Chao Wang; Yue Yan; Mengshan Zhang; Kevin H. Mayo; Lin Sun; Yifa Zhou

doi:10.1016/j.carres.2023.108895

An efficient protocol for preparing linear β-manno-oligosaccharides

Liangnan Cui, Xiang Wang, Chao Wang, Yue Yan, Mengshan Zhang, Kevin H. Mayo, Lin Sun, Yifa Zhou

Chemical and Structural Biology (TMED)

Research output: Contribution to journal › Article › peer-review

2 Scopus citations

Abstract

Linear β-manno-oligosaccharides (L-β-MOS) are widely used to investigate oligo- and poly-saccharide structures and mannanolytic enzyme activities. L-β-MOS are also being used as prebiotic agents with potential bio-active properties. In this study, we developed an efficient protocol to prepare a series of L-β-MOS by hydrolyzing cassia gum (CG) using mannanolytic enzymes (endo-1,4-β-mannanase, α-galactosidases and β-glucosidases). By using medium pressure liquid chromatography (MPLC), we purified L-β-MOS with different degrees of polymerization (DPs). HPAEC-PAD, MALDI-TOF-MS and NMR studies confirmed that these L-β-MOS species ranged from 1,4-β-D-mannobiose to 1,4-β-D-mannononaose (DP 2–9) with >95% purity. Our results provide a robust approach to preparing L-β-MOS, thus enabling L-β-MOS to be further used in the fields of chemistry, life science, and nutritional food.

Original language	English (US)
Article number	108895
Journal	Carbohydrate Research
Volume	532
DOIs	https://doi.org/10.1016/j.carres.2023.108895
State	Published - Oct 2023

Bibliographical note

Funding Information:
This work was supported by the National Natural Science Foundation of China (grant number: 32201051 ), the Funds from Jilin Province Development and Reform Commission (grant number: 2022C042-8) and the Fundamental Research Funds for the Central Universities (grant number: 2412021QD015 )

Publisher Copyright:
© 2023 Elsevier Ltd

Keywords

Cassia gum
Enzymatic hydrolysis
Manno-oligosaccharides
Purification

PubMed: MeSH publication types

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title = "An efficient protocol for preparing linear β-manno-oligosaccharides",

abstract = "Linear β-manno-oligosaccharides (L-β-MOS) are widely used to investigate oligo- and poly-saccharide structures and mannanolytic enzyme activities. L-β-MOS are also being used as prebiotic agents with potential bio-active properties. In this study, we developed an efficient protocol to prepare a series of L-β-MOS by hydrolyzing cassia gum (CG) using mannanolytic enzymes (endo-1,4-β-mannanase, α-galactosidases and β-glucosidases). By using medium pressure liquid chromatography (MPLC), we purified L-β-MOS with different degrees of polymerization (DPs). HPAEC-PAD, MALDI-TOF-MS and NMR studies confirmed that these L-β-MOS species ranged from 1,4-β-D-mannobiose to 1,4-β-D-mannononaose (DP 2–9) with >95% purity. Our results provide a robust approach to preparing L-β-MOS, thus enabling L-β-MOS to be further used in the fields of chemistry, life science, and nutritional food.",

keywords = "Cassia gum, Enzymatic hydrolysis, Manno-oligosaccharides, Purification",

author = "Liangnan Cui and Xiang Wang and Chao Wang and Yue Yan and Mengshan Zhang and Mayo, {Kevin H.} and Lin Sun and Yifa Zhou",

note = "Funding Information: This work was supported by the National Natural Science Foundation of China (grant number: 32201051 ), the Funds from Jilin Province Development and Reform Commission (grant number: 2022C042-8) and the Fundamental Research Funds for the Central Universities (grant number: 2412021QD015 ) Publisher Copyright: {\textcopyright} 2023 Elsevier Ltd",

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journal = "Carbohydrate Research",

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AU - Cui, Liangnan

AU - Wang, Xiang

AU - Wang, Chao

AU - Yan, Yue

AU - Zhang, Mengshan

AU - Mayo, Kevin H.

AU - Sun, Lin

AU - Zhou, Yifa

N1 - Funding Information: This work was supported by the National Natural Science Foundation of China (grant number: 32201051 ), the Funds from Jilin Province Development and Reform Commission (grant number: 2022C042-8) and the Fundamental Research Funds for the Central Universities (grant number: 2412021QD015 ) Publisher Copyright: © 2023 Elsevier Ltd

PY - 2023/10

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N2 - Linear β-manno-oligosaccharides (L-β-MOS) are widely used to investigate oligo- and poly-saccharide structures and mannanolytic enzyme activities. L-β-MOS are also being used as prebiotic agents with potential bio-active properties. In this study, we developed an efficient protocol to prepare a series of L-β-MOS by hydrolyzing cassia gum (CG) using mannanolytic enzymes (endo-1,4-β-mannanase, α-galactosidases and β-glucosidases). By using medium pressure liquid chromatography (MPLC), we purified L-β-MOS with different degrees of polymerization (DPs). HPAEC-PAD, MALDI-TOF-MS and NMR studies confirmed that these L-β-MOS species ranged from 1,4-β-D-mannobiose to 1,4-β-D-mannononaose (DP 2–9) with >95% purity. Our results provide a robust approach to preparing L-β-MOS, thus enabling L-β-MOS to be further used in the fields of chemistry, life science, and nutritional food.

AB - Linear β-manno-oligosaccharides (L-β-MOS) are widely used to investigate oligo- and poly-saccharide structures and mannanolytic enzyme activities. L-β-MOS are also being used as prebiotic agents with potential bio-active properties. In this study, we developed an efficient protocol to prepare a series of L-β-MOS by hydrolyzing cassia gum (CG) using mannanolytic enzymes (endo-1,4-β-mannanase, α-galactosidases and β-glucosidases). By using medium pressure liquid chromatography (MPLC), we purified L-β-MOS with different degrees of polymerization (DPs). HPAEC-PAD, MALDI-TOF-MS and NMR studies confirmed that these L-β-MOS species ranged from 1,4-β-D-mannobiose to 1,4-β-D-mannononaose (DP 2–9) with >95% purity. Our results provide a robust approach to preparing L-β-MOS, thus enabling L-β-MOS to be further used in the fields of chemistry, life science, and nutritional food.

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An efficient protocol for preparing linear β-manno-oligosaccharides

Abstract

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