Abstract
It is important to know the contribution of specific allergens to a complex allergenic extract and to have a dependable method to assess the effector activity of an extract specifically depleted of that allergen. We have previously shown that removal of the major peanut allergen, Ara h 2, from a crude peanut extract (CPE) minimally altered the effector activity of the extract. Here we describe in detail the methodology used to generate specific rabbit anti-peptide antibodies to remove a related peanut allergen, Ara h 6, from CPE and describe an improvement in the RBL SX-38 cell assay used to assess the effector activity of treated extracts. Our results show that although Ara h 2 and Ara h 6 can be selectively removed from a CPE, removal of each alone from a CPE had no significant effect on the effector activity. However, removal of Ara h 2 and Ara h 6 together significantly reduced the effector activity of CPE.
Original language | English (US) |
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Pages (from-to) | 65-70 |
Number of pages | 6 |
Journal | Journal of Immunological Methods |
Volume | 372 |
Issue number | 1-2 |
DOIs | |
State | Published - Sep 30 2011 |
Externally published | Yes |
Keywords
- Allergen
- Ara h 2
- Ara h 6
- IgE
- Peanut allergy
- Peanuts