Abstract
Purpose: The authors investigated the utility of attenuated Salmonella typhimurium for preventing the establishment of hepatic metastases in a murine model. Methods: A single, oral 108 cfu dose of attenuated S typhimurium was given 8 days before the establishment of a model of unresectable hepatic metastases. Animals were assessed for hepatic tumor number and volume, hepatic lymphocyte population analysis, and survival. Results: Pretreatment with Salmonella provided a 10-fold reduction in hepatic tumor burden compared with saline-treated controls. The antitumor effect is associated with markedly elevated natural killer (NK), CD8+ and CD4+ hepatic lymphocytes. Pretreatment with Salmonella provided a 90-day survival rate of 30%, whereas control animals were dead by 30 days. All long-term survivors were devoid of hepatic tumor. Conclusions: Attenuated S typhimurium effectively prevents the establishment of hepatic metastases in a murine model, providing a clear survival benefit. Thus, it may represent a novel form of in vivo immunotherapy for the prevention of hepatic metastases for patients with locally advanced colorectal cancer.
Original language | English (US) |
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Pages (from-to) | 1075-1079 |
Number of pages | 5 |
Journal | Journal of Pediatric Surgery |
Volume | 38 |
Issue number | 7 |
DOIs | |
State | Published - Jul 1 2003 |
Bibliographical note
Funding Information:This study was supported through a grant from the Phileona Foundation.
Copyright:
Copyright 2017 Elsevier B.V., All rights reserved.
Keywords
- Cancer
- Metastases
- Prevention
- Salmonella