Bioaugmentation potential of a cold-adapted and nitrate-reducing fungus to enhance nitrate removal in woodchip bioreactors

Nour Aldossari; Satoshi Ishii

doi:10.1016/j.biteb.2022.100969

Bioaugmentation potential of a cold-adapted and nitrate-reducing fungus to enhance nitrate removal in woodchip bioreactors

Nour Aldossari, Satoshi Ishii

Soil, Water, and Climate

Research output: Contribution to journal › Article › peer-review

2 Scopus citations

Abstract

Woodchip bioreactors are a promising approach to remove nitrate from agricultural drainage systems; however, their low removal efficiency at cold temperatures remains a significant challenge. Here we analyzed the efficacy of fungal inoculation to enhance nitrate removal in woodchip bioreactor microcosms under cold conditions. Among the cold-adapted, nitrate-reducing, and cellulose-degrading fungi previously isolated from soil, we identified Linnemannia hyaline strain SCG-10 as the fastest nitrate reducer. Inoculation of this fungal strain enhanced the rates of nitrate reduction, N₂ production, and dissolved organic carbon production in woodchip bioreactor microcosms at 5 °C. Production of N₂O was minor (<1%) in this study most likely due to the enhanced activity of N₂O reducers by labile carbon. Quantitative PCR for nitrogen cycle-related genes showed the active transcription of both fungal and bacterial denitrification genes. These results suggest that inoculating cold-adapted, nitrate-reducing, and cellulose-degrading fungi is a promising approach to remove nitrate from agricultural fields under cold conditions.

Original language	English (US)
Article number	100969
Journal	Bioresource Technology Reports
Volume	17
DOIs	https://doi.org/10.1016/j.biteb.2022.100969
State	Published - Feb 2022

Bibliographical note

Funding Information:
We thank Hao Wang for the technical assistance with the NiCE chip. We also thank Dr. Brandy Toner at the University of Minnesota for her support and encouragement to complete this study and Dr. Gary Feyereisen at the USDA-ARS for his help collecting woodchip samples. This research was supported by the MnDRIVE Initiative of the University of Minnesota . AD was supported by the Saudi Arabian Cultural Mission Scholarship and the Minnesota Mycological Society Scholarship.

Publisher Copyright:
© 2022 Elsevier Ltd

Keywords

Co-denitrification
Denitrification
Fungi
Quantitative PCR
Woodchip bioreactor

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abstract = "Woodchip bioreactors are a promising approach to remove nitrate from agricultural drainage systems; however, their low removal efficiency at cold temperatures remains a significant challenge. Here we analyzed the efficacy of fungal inoculation to enhance nitrate removal in woodchip bioreactor microcosms under cold conditions. Among the cold-adapted, nitrate-reducing, and cellulose-degrading fungi previously isolated from soil, we identified Linnemannia hyaline strain SCG-10 as the fastest nitrate reducer. Inoculation of this fungal strain enhanced the rates of nitrate reduction, N2 production, and dissolved organic carbon production in woodchip bioreactor microcosms at 5 °C. Production of N2O was minor (<1%) in this study most likely due to the enhanced activity of N2O reducers by labile carbon. Quantitative PCR for nitrogen cycle-related genes showed the active transcription of both fungal and bacterial denitrification genes. These results suggest that inoculating cold-adapted, nitrate-reducing, and cellulose-degrading fungi is a promising approach to remove nitrate from agricultural fields under cold conditions.",

keywords = "Co-denitrification, Denitrification, Fungi, Quantitative PCR, Woodchip bioreactor",

author = "Nour Aldossari and Satoshi Ishii",

note = "Funding Information: We thank Hao Wang for the technical assistance with the NiCE chip. We also thank Dr. Brandy Toner at the University of Minnesota for her support and encouragement to complete this study and Dr. Gary Feyereisen at the USDA-ARS for his help collecting woodchip samples. This research was supported by the MnDRIVE Initiative of the University of Minnesota . AD was supported by the Saudi Arabian Cultural Mission Scholarship and the Minnesota Mycological Society Scholarship. Publisher Copyright: {\textcopyright} 2022 Elsevier Ltd",

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AU - Ishii, Satoshi

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N2 - Woodchip bioreactors are a promising approach to remove nitrate from agricultural drainage systems; however, their low removal efficiency at cold temperatures remains a significant challenge. Here we analyzed the efficacy of fungal inoculation to enhance nitrate removal in woodchip bioreactor microcosms under cold conditions. Among the cold-adapted, nitrate-reducing, and cellulose-degrading fungi previously isolated from soil, we identified Linnemannia hyaline strain SCG-10 as the fastest nitrate reducer. Inoculation of this fungal strain enhanced the rates of nitrate reduction, N2 production, and dissolved organic carbon production in woodchip bioreactor microcosms at 5 °C. Production of N2O was minor (<1%) in this study most likely due to the enhanced activity of N2O reducers by labile carbon. Quantitative PCR for nitrogen cycle-related genes showed the active transcription of both fungal and bacterial denitrification genes. These results suggest that inoculating cold-adapted, nitrate-reducing, and cellulose-degrading fungi is a promising approach to remove nitrate from agricultural fields under cold conditions.

AB - Woodchip bioreactors are a promising approach to remove nitrate from agricultural drainage systems; however, their low removal efficiency at cold temperatures remains a significant challenge. Here we analyzed the efficacy of fungal inoculation to enhance nitrate removal in woodchip bioreactor microcosms under cold conditions. Among the cold-adapted, nitrate-reducing, and cellulose-degrading fungi previously isolated from soil, we identified Linnemannia hyaline strain SCG-10 as the fastest nitrate reducer. Inoculation of this fungal strain enhanced the rates of nitrate reduction, N2 production, and dissolved organic carbon production in woodchip bioreactor microcosms at 5 °C. Production of N2O was minor (<1%) in this study most likely due to the enhanced activity of N2O reducers by labile carbon. Quantitative PCR for nitrogen cycle-related genes showed the active transcription of both fungal and bacterial denitrification genes. These results suggest that inoculating cold-adapted, nitrate-reducing, and cellulose-degrading fungi is a promising approach to remove nitrate from agricultural fields under cold conditions.

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KW - Quantitative PCR

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Bioaugmentation potential of a cold-adapted and nitrate-reducing fungus to enhance nitrate removal in woodchip bioreactors

Abstract

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Keywords

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