Abstract
Biological synthesis of plant secondary metabolites has attracted increasing attention due to their proven or assumed beneWcial properties and health-promoting eVects. Phenylpropanoids are the precursors to a range of important plant metabolites such as the secondary metabolites belonging to the Xavonoid/stilbenoid class of compounds. In this study, engineered Escherichia coli containing artiWcial phenylpropanoid biosynthetic pathways utilizing tyrosine as the initial precursor were established for production of plant-speciWc metabolites such as ferulic acid, naringenin, and resveratrol. The construction of the artiWcial pathway utilized tyrosine ammonia lyase and 4-coumarate 3-hydroxylase from Saccharothrix espanaensis, cinnamate/4-coumarate: coenzyme A ligase from Streptomyces coelicolor, caVeic acid O-methyltransferase and chalcone synthase from Arabidopsis thaliana, and stilbene synthase from Arachis hypogaea.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 1657-1665 |
| Number of pages | 9 |
| Journal | Journal of Industrial Microbiology and Biotechnology |
| Volume | 38 |
| Issue number | 10 |
| DOIs | |
| State | Published - Oct 2011 |
| Externally published | Yes |
Bibliographical note
Funding Information:This work was supported in part by the 21C Frontier Microbial Genomics and Application Center, Basic Science Research Program, and Global R&D Center program, the Ministry of Science and Technology, Republic of Korea, and by a grant from KRIBB Research Initiative Program. The authors would also like to thank Joon-Tae Park for assistance in the LC–MS experiment.
Keywords
- ArtiWcial pathway
- Biosynthesis
- Heterologous expression
- Phenylpropanoid