Capillary electrophoresis for the investigation of prostate-specific antigen heterogeneity

Maura J. Donohue; Mary B. Satterfield; Joseph J. Dalluge; Michael J. Welch; James E. Girard; David M. Bunk

doi:10.1016/j.ab.2005.01.043

Capillary electrophoresis for the investigation of prostate-specific antigen heterogeneity

Maura J. Donohue, Mary B. Satterfield, Joseph J. Dalluge, Michael J. Welch, James E. Girard, David M. Bunk

Research output: Contribution to journal › Article › peer-review

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Abstract

Prostate-specific antigen (PSA) is a single-chain glycoprotein that is used as a biomarker for prostate-related diseases. PSA has one known posttranslational modification, a sialylated diantennary N-linked oligosaccharide attached to the asparagine residue N45. In this study capillary electrophoresis (CE) was employed to separate the isoforms of seven commercially available free PSA samples, two of which were specialized: enzymatically active PSA and noncomplexing PSA. The free PSA samples examined migrated as four to nine distinct, highly resolved peaks, indicating the presence of several isoforms differing in their oligosaccharide compositions. Overall, the use of CE provides a rapid, reproducible method for separation of PSA into its individual isoforms.

Original language	English (US)
Pages (from-to)	318-327
Number of pages	10
Journal	Analytical Biochemistry
Volume	339
Issue number	2
DOIs	https://doi.org/10.1016/j.ab.2005.01.043
State	Published - Apr 15 2005
Externally published	Yes

Bibliographical note

Funding Information:
MJD thanks the National Science Foundation for the Chemometrics Fellowship and the National Institute of Standards and Technology for their support of this research.

Keywords

Capillary electrophoresis
Heterogeneity
Isoforms
Prostate-specific antigen

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title = "Capillary electrophoresis for the investigation of prostate-specific antigen heterogeneity",

abstract = "Prostate-specific antigen (PSA) is a single-chain glycoprotein that is used as a biomarker for prostate-related diseases. PSA has one known posttranslational modification, a sialylated diantennary N-linked oligosaccharide attached to the asparagine residue N45. In this study capillary electrophoresis (CE) was employed to separate the isoforms of seven commercially available free PSA samples, two of which were specialized: enzymatically active PSA and noncomplexing PSA. The free PSA samples examined migrated as four to nine distinct, highly resolved peaks, indicating the presence of several isoforms differing in their oligosaccharide compositions. Overall, the use of CE provides a rapid, reproducible method for separation of PSA into its individual isoforms.",

keywords = "Capillary electrophoresis, Heterogeneity, Isoforms, Prostate-specific antigen",

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note = "Funding Information: MJD thanks the National Science Foundation for the Chemometrics Fellowship and the National Institute of Standards and Technology for their support of this research.",

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AU - Donohue, Maura J.

AU - Satterfield, Mary B.

AU - Dalluge, Joseph J.

AU - Welch, Michael J.

AU - Girard, James E.

AU - Bunk, David M.

N1 - Funding Information: MJD thanks the National Science Foundation for the Chemometrics Fellowship and the National Institute of Standards and Technology for their support of this research.

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AB - Prostate-specific antigen (PSA) is a single-chain glycoprotein that is used as a biomarker for prostate-related diseases. PSA has one known posttranslational modification, a sialylated diantennary N-linked oligosaccharide attached to the asparagine residue N45. In this study capillary electrophoresis (CE) was employed to separate the isoforms of seven commercially available free PSA samples, two of which were specialized: enzymatically active PSA and noncomplexing PSA. The free PSA samples examined migrated as four to nine distinct, highly resolved peaks, indicating the presence of several isoforms differing in their oligosaccharide compositions. Overall, the use of CE provides a rapid, reproducible method for separation of PSA into its individual isoforms.

KW - Capillary electrophoresis

KW - Heterogeneity

KW - Isoforms

KW - Prostate-specific antigen

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Capillary electrophoresis for the investigation of prostate-specific antigen heterogeneity

Abstract

Bibliographical note

Keywords

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