Cell cycle dependent resistance to staphylococcal delta toxin-induced lysis of cultured cells

Jon P. Durkin; W. Thomas Shier

doi:10.1016/0006-291X(80)91331-5

Cell cycle dependent resistance to staphylococcal delta toxin-induced lysis of cultured cells

Jon P. Durkin, W. Thomas Shier

Medicinal Chemistry

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Abstract

Staphylococcal delta toxin is a protein capable of rapidly disrupting cell membranes. Synchronized populations of 3T3 mouse fibroblasts in mitosis and early G₁ phases of the cell cycle exhibit resistance to delta toxin at concentrations cytolytic to interphase cells. Similar results were obtained with HeLa cells grown attached or in suspension culture. Increased resistance appears to result from structural or biochemical features other than cell rounding or detachment. Delta toxin stimulated significantly less cellular phospholipase A₂ (a potentially lytic enzyme activity) in mitotic 3T3 cells than in interphase cells.

Original language	English (US)
Pages (from-to)	980-987
Number of pages	8
Journal	Biochemical and Biophysical Research Communications
Volume	94
Issue number	3
DOIs	https://doi.org/10.1016/0006-291X(80)91331-5
State	Published - Jun 16 1980

Bibliographical note

Funding Information:
ACKNOWLEDGMENTS: Dr. A.W. Bernheimer; and the technical by National Cancer National Research We gratefully acknowledge a gift of delta toxin from a gift of mithramycin from Mr. N. Belcher, Pfizer Inc.; assistance of Mr. J. T. Trotter. This work was supported Institute, USPHS, the Cystic Fibrosis Foundation and the Council Canada.

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title = "Cell cycle dependent resistance to staphylococcal delta toxin-induced lysis of cultured cells",

abstract = "Staphylococcal delta toxin is a protein capable of rapidly disrupting cell membranes. Synchronized populations of 3T3 mouse fibroblasts in mitosis and early G1 phases of the cell cycle exhibit resistance to delta toxin at concentrations cytolytic to interphase cells. Similar results were obtained with HeLa cells grown attached or in suspension culture. Increased resistance appears to result from structural or biochemical features other than cell rounding or detachment. Delta toxin stimulated significantly less cellular phospholipase A2 (a potentially lytic enzyme activity) in mitotic 3T3 cells than in interphase cells.",

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Cell cycle dependent resistance to staphylococcal delta toxin-induced lysis of cultured cells

Abstract

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