TY - JOUR
T1 - Cellular chirality arising from the self-organization of the actin cytoskeleton
AU - Tee, Yee Han
AU - Shemesh, Tom
AU - Thiagarajan, Visalatchi
AU - Hariadi, Rizal Fajar
AU - Anderson, Karen L.
AU - Page, Christopher
AU - Volkmann, Niels
AU - Hanein, Dorit
AU - Sivaramakrishnan, Sivaraj
AU - Kozlov, Michael M.
AU - Bershadsky, Alexander D.
N1 - Publisher Copyright:
© 2015 Macmillan Publishers Limited.
PY - 2015/4/30
Y1 - 2015/4/30
N2 - Cellular mechanisms underlying the development of left-right asymmetry in tissues and embryos remain obscure. Here, the development of a chiral pattern of actomyosin was revealed by studying actin cytoskeleton self-organization in cells with isotropic circular shape. A radially symmetrical system of actin bundles consisting of α-actinin-enriched radial fibres (RFs) and myosin-IIA-enriched transverse fibres (TFs) evolved spontaneously into the chiral system as a result of the unidirectional tilting of all RFs, which was accompanied by a tangential shift in the retrograde movement of TFs. We showed that myosin-IIA-dependent contractile stresses within TFs drive their movement along RFs, which grow centripetally in a formin-dependent fashion. The handedness of the chiral pattern was shown to be regulated by α-actinin-1. Computational modelling demonstrated that the dynamics of the RF-TF system can explain the pattern transition from radial to chiral. Thus, actin cytoskeleton self-organization provides built-in machinery that potentially allows cells to develop left-right asymmetry.
AB - Cellular mechanisms underlying the development of left-right asymmetry in tissues and embryos remain obscure. Here, the development of a chiral pattern of actomyosin was revealed by studying actin cytoskeleton self-organization in cells with isotropic circular shape. A radially symmetrical system of actin bundles consisting of α-actinin-enriched radial fibres (RFs) and myosin-IIA-enriched transverse fibres (TFs) evolved spontaneously into the chiral system as a result of the unidirectional tilting of all RFs, which was accompanied by a tangential shift in the retrograde movement of TFs. We showed that myosin-IIA-dependent contractile stresses within TFs drive their movement along RFs, which grow centripetally in a formin-dependent fashion. The handedness of the chiral pattern was shown to be regulated by α-actinin-1. Computational modelling demonstrated that the dynamics of the RF-TF system can explain the pattern transition from radial to chiral. Thus, actin cytoskeleton self-organization provides built-in machinery that potentially allows cells to develop left-right asymmetry.
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U2 - 10.1038/ncb3137
DO - 10.1038/ncb3137
M3 - Article
C2 - 25799062
AN - SCOPUS:84925945544
SN - 1465-7392
VL - 17
SP - 445
EP - 457
JO - Nature Cell Biology
JF - Nature Cell Biology
IS - 4
ER -