Characterization of an Anther- and Tapetum-specific Gene Encoding a Glycine-rich Protein from Tomato

Ridong Chen; Penny J. Aguirre; Alan G. Smith

doi:10.1016/S0176-1617(11)81153-3

Characterization of an Anther- and Tapetum-specific Gene Encoding a Glycine-rich Protein from Tomato

Ridong Chen, Penny J. Aguirre, Alan G. Smith

Horticultural Science

Research output: Contribution to journal › Article › peer-review

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Abstract

A eDNA clone, TomA92B9, encoding a putative 15-kDa glycine-rich protein from tomato anthers, was isolated and characterized. The gene is present in one copy per haploid genome. The mRNA accumulated predominantly in the tapetal layer of stamens from early meiosis through tetrad release. The protein deduced from this clone has an N-terminal hydrophobic domain with the characteristics of a secretory signal. The mature protein contains 32% of Gly residues. On the basis of the analysis of gene's expression pattern and its primary structure, it is concluded that this gene product is a novel glycine-rich protein.

Original language	English (US)
Pages (from-to)	651-658
Number of pages	8
Journal	Journal of Plant Physiology
Volume	143
Issue number	6
DOIs	https://doi.org/10.1016/S0176-1617(11)81153-3
State	Published - Jan 1 1994

Keywords

DNA complementary to RNA
Lycopersicon esculentum
RNase
SDS
base pair(s)
bp
cDNA sequence
eDNA
flower
kDa
kb
kilobase(s) or 1000 bp
kilodalton
localization
nt
nucleotide(s)
ribonuclease
sodium dodecyl sulfate
stamen

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title = "Characterization of an Anther- and Tapetum-specific Gene Encoding a Glycine-rich Protein from Tomato",

abstract = "A eDNA clone, TomA92B9, encoding a putative 15-kDa glycine-rich protein from tomato anthers, was isolated and characterized. The gene is present in one copy per haploid genome. The mRNA accumulated predominantly in the tapetal layer of stamens from early meiosis through tetrad release. The protein deduced from this clone has an N-terminal hydrophobic domain with the characteristics of a secretory signal. The mature protein contains 32% of Gly residues. On the basis of the analysis of gene's expression pattern and its primary structure, it is concluded that this gene product is a novel glycine-rich protein.",

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AU - Chen, Ridong

AU - Aguirre, Penny J.

AU - Smith, Alan G.

PY - 1994/1/1

Y1 - 1994/1/1

N2 - A eDNA clone, TomA92B9, encoding a putative 15-kDa glycine-rich protein from tomato anthers, was isolated and characterized. The gene is present in one copy per haploid genome. The mRNA accumulated predominantly in the tapetal layer of stamens from early meiosis through tetrad release. The protein deduced from this clone has an N-terminal hydrophobic domain with the characteristics of a secretory signal. The mature protein contains 32% of Gly residues. On the basis of the analysis of gene's expression pattern and its primary structure, it is concluded that this gene product is a novel glycine-rich protein.

AB - A eDNA clone, TomA92B9, encoding a putative 15-kDa glycine-rich protein from tomato anthers, was isolated and characterized. The gene is present in one copy per haploid genome. The mRNA accumulated predominantly in the tapetal layer of stamens from early meiosis through tetrad release. The protein deduced from this clone has an N-terminal hydrophobic domain with the characteristics of a secretory signal. The mature protein contains 32% of Gly residues. On the basis of the analysis of gene's expression pattern and its primary structure, it is concluded that this gene product is a novel glycine-rich protein.

KW - DNA complementary to RNA

KW - Lycopersicon esculentum

KW - RNase

KW - SDS

KW - base pair(s)

KW - bp

KW - cDNA sequence

KW - eDNA

KW - flower

KW - kDa

KW - kb

KW - kilobase(s) or 1000 bp

KW - kilodalton

KW - localization

KW - nt

KW - nucleotide(s)

KW - ribonuclease

KW - sodium dodecyl sulfate

KW - stamen

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JO - Journal of Plant Physiology

JF - Journal of Plant Physiology

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ER -

Characterization of an Anther- and Tapetum-specific Gene Encoding a Glycine-rich Protein from Tomato

Abstract

Keywords

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