Characterization of anti-porcine epidemic diarrhea virus neutralizing activity in mammary secretions

Qinye Song; Suzanne Stone; Donna Drebes; Laura L. Greiner; Cheryl M.T. Dvorak; Michael P. Murtaugh

doi:10.1016/j.virusres.2016.06.002

Characterization of anti-porcine epidemic diarrhea virus neutralizing activity in mammary secretions

Qinye Song, Suzanne Stone, Donna Drebes, Laura L. Greiner, Cheryl M.T. Dvorak, Michael P. Murtaugh

Veterinary and Biomedical Sciences

Research output: Contribution to journal › Article › peer-review

30 Scopus citations

Abstract

Porcine epidemic diarrhea virus (PEDV) causes a severe clinical enteric disease in suckling neonates with up to 100% mortality, resulting in devastating economic losses to the pork industry in recent years. Maternal immunity via colostrum and milk is a vital source to neonates of passive protection against diarrhea, dehydration and death caused by PEDV. Comprehensive information on neutralizing activity (NA) against PEDV in mammary secretions is critically important for assessing the protective capacity of sows. Therefore, the objectives of this study were to characterize anti-PEDV neutralizing activity in mammary secretions. Anti-PEDV NA was present in colostrum, milk and serum from PEDV-infected sows as determined both by immunofluorescence and ELISA-based neutralizing assays, with neutralization levels higher in colostrum and milk than in serum. The highest NA was observed in colostrum on day 1, and decreased rapidly in milk at day 3, then gradually declined from day 3 to day 19 post-farrowing. Notably, the NA in mammary secretions showed various patterns of decline over time of lactation that may contribute to variation in sow protective capacities. The kinetics of NA decline were associated with total IgA and IgG antibody levels. Neutralizing activity significantly correlated with specific IgA primarily to spike domain 1 (S1) and domain 2 (S2) proteins of PEDV rather than to specific IgG in colostrum. Subsequently, the NA in milk was mainly related to specific IgA to S1 and S2 during lactation.

Original language	English (US)
Pages (from-to)	85-92
Number of pages	8
Journal	Virus research
Volume	226
DOIs	https://doi.org/10.1016/j.virusres.2016.06.002
State	Published - Dec 2 2016

Bibliographical note

Funding Information:
This work was supported by the Cooperative State Research Service , U.S. Department of Agriculture, under project MIN-63-112, National Pork Board grant 14-175, the China Scholarship Council and the National Natural Science Foundation of China ( 31372441 ) to support QS, and Carthage Veterinary Services, Carthage, IL. DD was a DVM student in the College of Veterinary Medicine, University of Minnesota, sponsored by Carthage Veterinary Services on an internship. We thank Audrey Tseng, an undergraduate student at the University of Minnesota, for assistance in protein expression and purification.

Publisher Copyright:
© 2016 Elsevier B.V.

Keywords

Anti-PEDV IgA
Colostrum and milk
Correlation
Dynamics
Neutralizing activity
PEDV

Access

10.1016/j.virusres.2016.06.002

OpenUrl availability

Full text

Cite this

@article{bf068a02e68e4cc99186706ac7855d79,

title = "Characterization of anti-porcine epidemic diarrhea virus neutralizing activity in mammary secretions",

abstract = "Porcine epidemic diarrhea virus (PEDV) causes a severe clinical enteric disease in suckling neonates with up to 100% mortality, resulting in devastating economic losses to the pork industry in recent years. Maternal immunity via colostrum and milk is a vital source to neonates of passive protection against diarrhea, dehydration and death caused by PEDV. Comprehensive information on neutralizing activity (NA) against PEDV in mammary secretions is critically important for assessing the protective capacity of sows. Therefore, the objectives of this study were to characterize anti-PEDV neutralizing activity in mammary secretions. Anti-PEDV NA was present in colostrum, milk and serum from PEDV-infected sows as determined both by immunofluorescence and ELISA-based neutralizing assays, with neutralization levels higher in colostrum and milk than in serum. The highest NA was observed in colostrum on day 1, and decreased rapidly in milk at day 3, then gradually declined from day 3 to day 19 post-farrowing. Notably, the NA in mammary secretions showed various patterns of decline over time of lactation that may contribute to variation in sow protective capacities. The kinetics of NA decline were associated with total IgA and IgG antibody levels. Neutralizing activity significantly correlated with specific IgA primarily to spike domain 1 (S1) and domain 2 (S2) proteins of PEDV rather than to specific IgG in colostrum. Subsequently, the NA in milk was mainly related to specific IgA to S1 and S2 during lactation.",

keywords = "Anti-PEDV IgA, Colostrum and milk, Correlation, Dynamics, Neutralizing activity, PEDV",

author = "Qinye Song and Suzanne Stone and Donna Drebes and Greiner, {Laura L.} and Dvorak, {Cheryl M.T.} and Murtaugh, {Michael P.}",

note = "Funding Information: This work was supported by the Cooperative State Research Service , U.S. Department of Agriculture, under project MIN-63-112, National Pork Board grant 14-175, the China Scholarship Council and the National Natural Science Foundation of China ( 31372441 ) to support QS, and Carthage Veterinary Services, Carthage, IL. DD was a DVM student in the College of Veterinary Medicine, University of Minnesota, sponsored by Carthage Veterinary Services on an internship. We thank Audrey Tseng, an undergraduate student at the University of Minnesota, for assistance in protein expression and purification. Publisher Copyright: {\textcopyright} 2016 Elsevier B.V.",

year = "2016",

month = dec,

day = "2",

doi = "10.1016/j.virusres.2016.06.002",

language = "English (US)",

volume = "226",

pages = "85--92",

journal = "Virus research",

issn = "0168-1702",

publisher = "Elsevier",

}

TY - JOUR

T1 - Characterization of anti-porcine epidemic diarrhea virus neutralizing activity in mammary secretions

AU - Song, Qinye

AU - Stone, Suzanne

AU - Drebes, Donna

AU - Greiner, Laura L.

AU - Dvorak, Cheryl M.T.

AU - Murtaugh, Michael P.

N1 - Funding Information: This work was supported by the Cooperative State Research Service , U.S. Department of Agriculture, under project MIN-63-112, National Pork Board grant 14-175, the China Scholarship Council and the National Natural Science Foundation of China ( 31372441 ) to support QS, and Carthage Veterinary Services, Carthage, IL. DD was a DVM student in the College of Veterinary Medicine, University of Minnesota, sponsored by Carthage Veterinary Services on an internship. We thank Audrey Tseng, an undergraduate student at the University of Minnesota, for assistance in protein expression and purification. Publisher Copyright: © 2016 Elsevier B.V.

PY - 2016/12/2

Y1 - 2016/12/2

N2 - Porcine epidemic diarrhea virus (PEDV) causes a severe clinical enteric disease in suckling neonates with up to 100% mortality, resulting in devastating economic losses to the pork industry in recent years. Maternal immunity via colostrum and milk is a vital source to neonates of passive protection against diarrhea, dehydration and death caused by PEDV. Comprehensive information on neutralizing activity (NA) against PEDV in mammary secretions is critically important for assessing the protective capacity of sows. Therefore, the objectives of this study were to characterize anti-PEDV neutralizing activity in mammary secretions. Anti-PEDV NA was present in colostrum, milk and serum from PEDV-infected sows as determined both by immunofluorescence and ELISA-based neutralizing assays, with neutralization levels higher in colostrum and milk than in serum. The highest NA was observed in colostrum on day 1, and decreased rapidly in milk at day 3, then gradually declined from day 3 to day 19 post-farrowing. Notably, the NA in mammary secretions showed various patterns of decline over time of lactation that may contribute to variation in sow protective capacities. The kinetics of NA decline were associated with total IgA and IgG antibody levels. Neutralizing activity significantly correlated with specific IgA primarily to spike domain 1 (S1) and domain 2 (S2) proteins of PEDV rather than to specific IgG in colostrum. Subsequently, the NA in milk was mainly related to specific IgA to S1 and S2 during lactation.

AB - Porcine epidemic diarrhea virus (PEDV) causes a severe clinical enteric disease in suckling neonates with up to 100% mortality, resulting in devastating economic losses to the pork industry in recent years. Maternal immunity via colostrum and milk is a vital source to neonates of passive protection against diarrhea, dehydration and death caused by PEDV. Comprehensive information on neutralizing activity (NA) against PEDV in mammary secretions is critically important for assessing the protective capacity of sows. Therefore, the objectives of this study were to characterize anti-PEDV neutralizing activity in mammary secretions. Anti-PEDV NA was present in colostrum, milk and serum from PEDV-infected sows as determined both by immunofluorescence and ELISA-based neutralizing assays, with neutralization levels higher in colostrum and milk than in serum. The highest NA was observed in colostrum on day 1, and decreased rapidly in milk at day 3, then gradually declined from day 3 to day 19 post-farrowing. Notably, the NA in mammary secretions showed various patterns of decline over time of lactation that may contribute to variation in sow protective capacities. The kinetics of NA decline were associated with total IgA and IgG antibody levels. Neutralizing activity significantly correlated with specific IgA primarily to spike domain 1 (S1) and domain 2 (S2) proteins of PEDV rather than to specific IgG in colostrum. Subsequently, the NA in milk was mainly related to specific IgA to S1 and S2 during lactation.

KW - Anti-PEDV IgA

KW - Colostrum and milk

KW - Correlation

KW - Dynamics

KW - Neutralizing activity

KW - PEDV

UR - http://www.scopus.com/inward/record.url?scp=84994319081&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84994319081&partnerID=8YFLogxK

U2 - 10.1016/j.virusres.2016.06.002

DO - 10.1016/j.virusres.2016.06.002

M3 - Article

C2 - 27287711

AN - SCOPUS:84994319081

SN - 0168-1702

VL - 226

SP - 85

EP - 92

JO - Virus research

JF - Virus research

ER -

Characterization of anti-porcine epidemic diarrhea virus neutralizing activity in mammary secretions

Abstract

Bibliographical note

Keywords

Access

OpenUrl availability

Other files and links

Fingerprint

Cite this