Abstract
A positive Mycoplasma hyopneumoniae PCR result in a clinical specimen may eventually represent the mere detection of non-viable bacteria, complicating the diagnostic interpretation. Thus, the objective of this study was to evaluate the PCR detection of non-viable M. hyopneumoniae and its residual cell-free DNA in live pigs. Pigs were inoculated with either active or inactivated M. hyopneumoniae and were sampled for up to 14 days. Mycoplasma hyopneumoniae was not detected by PCR at any timepoint in pigs inoculated with the inactivated bacterium, suggesting that in healthy pigs, the non-viable M. hyopneumoniae DNA was rapidly sensed and cleared.
Original language | English (US) |
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Pages (from-to) | 19 |
Number of pages | 1 |
Journal | Veterinary research |
Volume | 55 |
Issue number | 1 |
DOIs | |
State | Published - Feb 15 2024 |
Bibliographical note
Publisher Copyright:© 2024. The Author(s).
Keywords
- DNA
- Mycoplasma hyopneumoniae
- PCR
- bacterial DNA
- diagnostics
- pigs
PubMed: MeSH publication types
- Journal Article