TY - JOUR
T1 - Cholinergic Stimulation of Immunoglobulin A Secretion in Rat Intestine
AU - Wilson, I. Dodd
AU - Soltis, Ronald D.
AU - Olson, Robert E.
AU - Erlandsen, Stanley L.
PY - 1982
Y1 - 1982
N2 - This study was undertaken to assess a possible role for cholinergic agents in the regulation of intestinal immunoglobulin A secretion. Intestinal loops, constructed in anesthetized rats, were perf used with phosphate buffered normal saline. Immunoglobulin A concentrations were measured by radioimmunoassay. When compared with the effect of normal saline in the same rats, intravenous injection of pilocarpine, 10 mg/kg, increased immunoglobulin A concentrations in perf usates from ilea] loops (p < 0.001). Qualitatively similar results were obtained with muscarine and bethanechol, from jejunal and colonic loops, and from unanesthetized rats. Immunoglobulin A concentrations increased four- to eightf old during maximal cholinergic stimulation. Atropine, 250 μg intravenously, completely blocked the effect of pilocarpine on immunoglobulin A secretion (p < 0.005), and also inhibited basal intestinal immunoglobulin A secretion for 40 min after injection. As determined on 10%-40% sucrose density gradients, much of the immunoglobulin A secreted after cholinergic stimulation sedimented in the 11S range. These data indicate that intestinal secretion of immunoglobulin A is stimulated by the muscarinic effect of cholinergic agonists, and suggest that basal secretion of immunoglobulin A may be influenced by the parasympathetic nervous system.
AB - This study was undertaken to assess a possible role for cholinergic agents in the regulation of intestinal immunoglobulin A secretion. Intestinal loops, constructed in anesthetized rats, were perf used with phosphate buffered normal saline. Immunoglobulin A concentrations were measured by radioimmunoassay. When compared with the effect of normal saline in the same rats, intravenous injection of pilocarpine, 10 mg/kg, increased immunoglobulin A concentrations in perf usates from ilea] loops (p < 0.001). Qualitatively similar results were obtained with muscarine and bethanechol, from jejunal and colonic loops, and from unanesthetized rats. Immunoglobulin A concentrations increased four- to eightf old during maximal cholinergic stimulation. Atropine, 250 μg intravenously, completely blocked the effect of pilocarpine on immunoglobulin A secretion (p < 0.005), and also inhibited basal intestinal immunoglobulin A secretion for 40 min after injection. As determined on 10%-40% sucrose density gradients, much of the immunoglobulin A secreted after cholinergic stimulation sedimented in the 11S range. These data indicate that intestinal secretion of immunoglobulin A is stimulated by the muscarinic effect of cholinergic agonists, and suggest that basal secretion of immunoglobulin A may be influenced by the parasympathetic nervous system.
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U2 - 10.1016/S0016-5085(82)80020-6
DO - 10.1016/S0016-5085(82)80020-6
M3 - Article
C2 - 6125453
AN - SCOPUS:0019944701
SN - 0016-5085
VL - 83
SP - 881
EP - 888
JO - Gastroenterology
JF - Gastroenterology
IS - 4
ER -