Abstract
A cDNA encoding the catalytic core of a novel brain 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase isoenzyme was isolated from a λgt10 bovine brain library. This brain cDNA begins and ends in an open reading frame encoding a peptide of 476 amino acids. This peptide contains both the catalytic kinase and bisphosphatase domains and has an overall 65% and 67% identity with the bovine heart and liver isozymes, respectively, whereas the NH2 and COOH-termini are divergent. An active catalytic core brain bifunctional enzyme was expressed in E. coli using a T7 RNA polymerase-based expression system. These results support the presence of a distinct gene coding for the protein in bovine brain.
Original language | English (US) |
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Pages (from-to) | 1140-1148 |
Number of pages | 9 |
Journal | Biochemical and Biophysical Research Communications |
Volume | 209 |
Issue number | 3 |
DOIs | |
State | Published - Apr 26 1995 |
Externally published | Yes |
Bibliographical note
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