TY - JOUR
T1 - Cloning and promoter mapping of mouse κ opioid receptor gene
AU - Liu, Hsien Ching
AU - Lu, Shengli L.
AU - Augustin, Lance B.
AU - Felsheim, Roderick F.
AU - Chen, Hung Chun
AU - Loh, Horace H.
AU - Wei, Li Na
PY - 1995/4/17
Y1 - 1995/4/17
N2 - We have isolated mouse κ opioid receptor (KOR) genomic clones containing the complete 5′-untranslated region, a portion of the 3′-untranslated region, and its coding region. The mouse KOR gene spans at least 16 kb and contains at least 4 exons. Exon I encodes the major portion of 5′-untranslated sequence and transcription initiation is determined, by primer extension experiments, to reside at 3 major sites, located at 334, 340, and 716 nucleotides upstream from the translation initiation codon. No typical TATA or CAAT boxes can be identified within an approximately 1-kb upstream region. Exon II contains 271 nucleotides, including 14 nucleotides of 5′-untranslated sequence. Exon III contains 353 nucleotides and exon IV begins at amino acid position #204 and encodes the rest of the 3′-end sequence of the cloned cDNA. Based upon Northern blot analysis, KOR transcript is estimated to be approximately 6 kb in length.
AB - We have isolated mouse κ opioid receptor (KOR) genomic clones containing the complete 5′-untranslated region, a portion of the 3′-untranslated region, and its coding region. The mouse KOR gene spans at least 16 kb and contains at least 4 exons. Exon I encodes the major portion of 5′-untranslated sequence and transcription initiation is determined, by primer extension experiments, to reside at 3 major sites, located at 334, 340, and 716 nucleotides upstream from the translation initiation codon. No typical TATA or CAAT boxes can be identified within an approximately 1-kb upstream region. Exon II contains 271 nucleotides, including 14 nucleotides of 5′-untranslated sequence. Exon III contains 353 nucleotides and exon IV begins at amino acid position #204 and encodes the rest of the 3′-end sequence of the cloned cDNA. Based upon Northern blot analysis, KOR transcript is estimated to be approximately 6 kb in length.
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U2 - 10.1006/bbrc.1995.1547
DO - 10.1006/bbrc.1995.1547
M3 - Article
C2 - 7733933
AN - SCOPUS:0028964573
SN - 0006-291X
VL - 209
SP - 639
EP - 647
JO - Biochemical and Biophysical Research Communications
JF - Biochemical and Biophysical Research Communications
IS - 2
ER -