Collagenesis and elastogenesis in fibrin-based media-equivalents treated with TGF-ßl and Insulin

Erin D. Grassl; Jennifer L. Long; Jeffrey R. Ross; Brett C. Isenberg; Theodore R. Oegema; Robert T. Tranquillo

Collagenesis and elastogenesis in fibrin-based media-equivalents treated with TGF-ßl and Insulin

Erin D. Grassl, Jennifer L. Long, Jeffrey R. Ross, Brett C. Isenberg, Theodore R. Oegema, Robert T. Tranquillo

Biomedical Engineering

Research output: Contribution to conference › Paper › peer-review

Abstract

The neonatal smooth muscle cells (SMC) produced more collagen when seeded in fibrin gel rather than collagen gel. The quantity of cross-linked elastic fibers was enhanced by treatment with TGFβ1 and insulin. The When compared to collagen ME , the fibrin ME compacted to a greater extent and were both stronger and stiffer when cultured under the same conditions, having a tensile modulus and ultimate tensile strength (UTS). It was found that the use of soluble factors increased elastin production, as measured by alkaline extraction and amino acid analysis, 5-fold in fibrin gels, which was about 4 times greater than in collagen gels.

Original language	English (US)
Number of pages	1
State	Published - Dec 1 2002
Event	Third Smith and Nephew International Symposium - Translating Tissue Engineering into Products - Atlanta, GA, United States Duration: Oct 13 2002 → Oct 16 2002

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Other	Third Smith and Nephew International Symposium - Translating Tissue Engineering into Products
Country/Territory	United States
City	Atlanta, GA
Period	10/13/02 → 10/16/02

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Collagenesis and elastogenesis in fibrin-based media-equivalents treated with TGF-ßl and Insulin. / Grassl, Erin D.; Long, Jennifer L.; Ross, Jeffrey R. et al.
2002. Paper presented at Third Smith and Nephew International Symposium - Translating Tissue Engineering into Products, Atlanta, GA, United States.

Research output: Contribution to conference › Paper › peer-review

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abstract = "The neonatal smooth muscle cells (SMC) produced more collagen when seeded in fibrin gel rather than collagen gel. The quantity of cross-linked elastic fibers was enhanced by treatment with TGFβ1 and insulin. The When compared to collagen ME , the fibrin ME compacted to a greater extent and were both stronger and stiffer when cultured under the same conditions, having a tensile modulus and ultimate tensile strength (UTS). It was found that the use of soluble factors increased elastin production, as measured by alkaline extraction and amino acid analysis, 5-fold in fibrin gels, which was about 4 times greater than in collagen gels.",

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AU - Grassl, Erin D.

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AU - Ross, Jeffrey R.

AU - Isenberg, Brett C.

AU - Oegema, Theodore R.

AU - Tranquillo, Robert T.

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AB - The neonatal smooth muscle cells (SMC) produced more collagen when seeded in fibrin gel rather than collagen gel. The quantity of cross-linked elastic fibers was enhanced by treatment with TGFβ1 and insulin. The When compared to collagen ME , the fibrin ME compacted to a greater extent and were both stronger and stiffer when cultured under the same conditions, having a tensile modulus and ultimate tensile strength (UTS). It was found that the use of soluble factors increased elastin production, as measured by alkaline extraction and amino acid analysis, 5-fold in fibrin gels, which was about 4 times greater than in collagen gels.

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T2 - Third Smith and Nephew International Symposium - Translating Tissue Engineering into Products

Y2 - 13 October 2002 through 16 October 2002

ER -

Collagenesis and elastogenesis in fibrin-based media-equivalents treated with TGF-ßl and Insulin

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