Abstract
Water-soluble polysaccharides were isolated from the leaves and roots of Isatis indigotica Fort., and their structural features were studied and compared. One neutral polysaccharide fraction (WFIP-N) and three pectin fractions (WFIP-A-A, WFIP-A-B and WFIP-A-C) were obtained from the leaves, and one neutral polysaccharide fraction (WRIP-N) and two pectin fractions (WRIP-A-A and WRIP-A-B) were obtained from the roots. WFIP-A-B (Mw = 34.6 kDa) and WRIP-A-B (Mw = 29.9 kDa) were the major pectic polysaccharides. Monosaccharide composition, FT-IR, enzymatic hydrolysis, NMR and methylation analysis indicated that both WFIP-A-B and WRIP-A-B are composed of rhamnogalacturonan I (RG-I), rhamnogalacturonan II (RG-II) and homogalacturonan (HG) domains with mass ratios of 1.5:1.0:0.4 and 0.3:1.0:1.7, respectively. WFIP-A-B and WRIP-A-B were found to be rich in RG-I and HG domains, respectively, and mainly contained type II arabinogalactan (AG-II) and α-L-1,5-arabinan side chains, but those in WRIP-A-B were more numerous and longer. Our results provide structural features and differences between these polysaccharides which will help to elucidate their functional differences.
Original language | English (US) |
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Pages (from-to) | 642-652 |
Number of pages | 11 |
Journal | International Journal of Biological Macromolecules |
Volume | 206 |
DOIs | |
State | Published - May 1 2022 |
Bibliographical note
Funding Information:This work was supported by the National Natural Science Foundation of China ( 31870796 ), the Scientific and Technologic Foundation of Jilin Province ( 20200201004JC ), the Fundamental Research Funds for the Central Universities ( 2412020ZD001 ) and the Innovation Platform Project of Qinghai province ( 2021-ZJ-T02 ).
Publisher Copyright:
© 2022 Elsevier B.V.
Keywords
- Isatis indigotica Fort.
- Pectin
- Polysaccharides
- Structural analysis
PubMed: MeSH publication types
- Journal Article