TY - JOUR
T1 - Competence for assembly of sister chromatid cores is progressively acquired during S phase in mammalian cells
AU - Giménez-Abiân, Juan F.
AU - Clarke, Duncan J.
AU - De La Torre, Consuelo
AU - Giménez-Martín, Gonzalo
AU - Mullinger, Ann M.
AU - Downes, C. Stephen
AU - Johnson, Robert T.
N1 - Funding Information:
paign, the DGICYT, Spain (Project PB96-0909) and the EU (Project BI04-Cf96-0275). We also thank the CAM for a grant supporting J. F. Gimenez-Abian, and'the EMBO for a fellowship awarded to D. J. Clarke.
PY - 1999
Y1 - 1999
N2 - Condensed sister chromatids possess a protein scaffold or axial core to which loops of chromatin are attached. The sister cores are believed to be dynamic frameworks that function in the organization and condensation of chromatids. Chromosome structural proteins are implicated in the establishment of sister chromatid cohesion and in the maintenance of epigenetic phenomena. Both processes of templating are tightly linked to DNA replication itself. It is a question whether the structural basis of sister chromatid cores is templated during S phase. As cells proceed through the cell cycle, chromatid cores undergo changes in their protein composition. Cytologically, cores are first visualized at the start of prometaphase. Still, core assembly can be induced in G1 and G2 when interphase cells are fused with mitotic cells. In this study, we asked if chromatid cores are similarly able to assemble in S-phase cells. We find that the ability to assemble cores is transiently lost during local replication, then regained in chromosome regions shortly after they have been replicated. We propose that core templating occurs coincident with DNA replication and that the competence for the assembly of the sister chromatid cores is acquired shortly after passage of replication forks.
AB - Condensed sister chromatids possess a protein scaffold or axial core to which loops of chromatin are attached. The sister cores are believed to be dynamic frameworks that function in the organization and condensation of chromatids. Chromosome structural proteins are implicated in the establishment of sister chromatid cohesion and in the maintenance of epigenetic phenomena. Both processes of templating are tightly linked to DNA replication itself. It is a question whether the structural basis of sister chromatid cores is templated during S phase. As cells proceed through the cell cycle, chromatid cores undergo changes in their protein composition. Cytologically, cores are first visualized at the start of prometaphase. Still, core assembly can be induced in G1 and G2 when interphase cells are fused with mitotic cells. In this study, we asked if chromatid cores are similarly able to assemble in S-phase cells. We find that the ability to assemble cores is transiently lost during local replication, then regained in chromosome regions shortly after they have been replicated. We propose that core templating occurs coincident with DNA replication and that the competence for the assembly of the sister chromatid cores is acquired shortly after passage of replication forks.
KW - Cell fusion
KW - Core assembly
KW - Prematurely condensed chromosomes
KW - S phase
KW - Sister chromatid cores
UR - http://www.scopus.com/inward/record.url?scp=0343609288&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0343609288&partnerID=8YFLogxK
U2 - 10.1016/S0171-9335(99)80026-0
DO - 10.1016/S0171-9335(99)80026-0
M3 - Article
C2 - 10494867
AN - SCOPUS:0343609288
SN - 0171-9335
VL - 78
SP - 601
EP - 603
JO - European Journal of Cell Biology
JF - European Journal of Cell Biology
IS - 8
ER -