Complete genome comparison of porcine reproductive and respiratory syndrome virus parental and attenuated strains

Shishan Yuan; Daniel Mickelson; Michael P. Murtaugh; Kay S. Faaberg

doi:10.1016/S0168-1702(00)00250-1

Complete genome comparison of porcine reproductive and respiratory syndrome virus parental and attenuated strains

Shishan Yuan, Daniel Mickelson, Michael P. Murtaugh, Kay S. Faaberg

Veterinary and Biomedical Sciences

Research output: Contribution to journal › Article › peer-review

23 Scopus citations

Abstract

Two full-length porcine reproductive and respiratory syndrome virus (PRRSV) genomes, strain VR-2332 and its cell culture passaged descendent RespPRRS vaccine strain, were compared and analyzed in order to identify possible sites of attenuation. Of the 44 nucleotide changes, 13 resulted in conservative changes and 18 produced non-conservative changes. The results suggest that key amino acids in ORF1 may contribute to the phenotype of RespPRRS, which includes increased growth rate on MA-104 cells and decreased virulence in swine. The results provide a genetic basis for future manipulation of a PRRSV reverse genetics system.

Original language	English (US)
Pages (from-to)	99-110
Number of pages	12
Journal	Virus research
Volume	74
Issue number	1-2
DOIs	https://doi.org/10.1016/S0168-1702(00)00250-1
State	Published - 2001

Bibliographical note

Funding Information:
The authors wish to thank Chris Nelsen and Faith Klebs for excellent technical expertise. Boehringer Ingelheim Vetmedica, Inc., provided financial support for the research.

Keywords

Genome comparison
Porcine reproductive and respiratory syndrome virus (PRRSV)

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title = "Complete genome comparison of porcine reproductive and respiratory syndrome virus parental and attenuated strains",

abstract = "Two full-length porcine reproductive and respiratory syndrome virus (PRRSV) genomes, strain VR-2332 and its cell culture passaged descendent RespPRRS vaccine strain, were compared and analyzed in order to identify possible sites of attenuation. Of the 44 nucleotide changes, 13 resulted in conservative changes and 18 produced non-conservative changes. The results suggest that key amino acids in ORF1 may contribute to the phenotype of RespPRRS, which includes increased growth rate on MA-104 cells and decreased virulence in swine. The results provide a genetic basis for future manipulation of a PRRSV reverse genetics system.",

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AU - Mickelson, Daniel

AU - Murtaugh, Michael P.

AU - Faaberg, Kay S.

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N2 - Two full-length porcine reproductive and respiratory syndrome virus (PRRSV) genomes, strain VR-2332 and its cell culture passaged descendent RespPRRS vaccine strain, were compared and analyzed in order to identify possible sites of attenuation. Of the 44 nucleotide changes, 13 resulted in conservative changes and 18 produced non-conservative changes. The results suggest that key amino acids in ORF1 may contribute to the phenotype of RespPRRS, which includes increased growth rate on MA-104 cells and decreased virulence in swine. The results provide a genetic basis for future manipulation of a PRRSV reverse genetics system.

AB - Two full-length porcine reproductive and respiratory syndrome virus (PRRSV) genomes, strain VR-2332 and its cell culture passaged descendent RespPRRS vaccine strain, were compared and analyzed in order to identify possible sites of attenuation. Of the 44 nucleotide changes, 13 resulted in conservative changes and 18 produced non-conservative changes. The results suggest that key amino acids in ORF1 may contribute to the phenotype of RespPRRS, which includes increased growth rate on MA-104 cells and decreased virulence in swine. The results provide a genetic basis for future manipulation of a PRRSV reverse genetics system.

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Complete genome comparison of porcine reproductive and respiratory syndrome virus parental and attenuated strains

Abstract

Bibliographical note

Keywords

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