Complete genome sequence of Methanospirillum hungatei type strain JF1

Robert P. Gunsalus; Lauren E. Cook; Bryan Crable; Lars Rohlin; Erin McDonald; Housna Mouttaki; Jessica R. Sieber; Nicole Poweleit; Hong Zhou; Alla L. Lapidus; Hajnalka Erzsebet Daligault; Miriam Land; Paul Gilna; Natalia Ivanova; Nikos Kyrpides; David E. Culley; Michael J. McInerney

doi:10.1186/s40793-015-0124-8

Complete genome sequence of Methanospirillum hungatei type strain JF1

Robert P. Gunsalus, Lauren E. Cook, Bryan Crable, Lars Rohlin, Erin McDonald, Housna Mouttaki, Jessica R. Sieber, Nicole Poweleit, Hong Zhou, Alla L. Lapidus, Hajnalka Erzsebet Daligault, Miriam Land, Paul Gilna, Natalia Ivanova, Nikos Kyrpides, David E. Culley, Michael J. McInerney

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Abstract

Methanospirillum hungatei strain JF1 (DSM 864) is a methane-producing archaeon and is the type species of the genus Methanospirillum, which belongs to the family Methanospirillaceae within the order Methanomicrobiales. Its genome was selected for sequencing due to its ability to utilize hydrogen and carbon dioxide and/or formate as a sole source of energy. Ecologically, M. hungatei functions as the hydrogen- and/or formate-using partner with many species of syntrophic bacteria. Its morphology is distinct from other methanogens with the ability to form long chains of cells (up to 100 μm in length), which are enclosed within a sheath-like structure, and terminal cells with polar flagella. The genome of M. hungatei strain JF1 is the first completely sequenced genome of the family Methanospirillaceae, and it has a circular genome of 3,544,738bp containing 3,239 protein coding and 68 RNA genes. The large genome of M. hungatei JF1 suggests the presence of unrecognized biochemical/physiological properties that likely extend to the other Methanospirillaceae and include the ability to form the unusual sheath-like structure and to successfully interact with syntrophic bacteria.

Original language	English (US)
Article number	2
Journal	Standards in Genomic Sciences
Volume	11
Issue number	1
DOIs	https://doi.org/10.1186/s40793-015-0124-8
State	Published - Jan 6 2016
Externally published	Yes

Bibliographical note

Funding Information:
This work was performed under the auspices of the US Department of Energy Office of Science Biological and Environmental Research Program. We also acknowledge support by the US Department of Energy Contract No. DE-FG02-96ER20214, from the Chemical Sciences, Geosciences and Biosciences Division, Office of Basic Energy Sciences (MJM) for syntrophy functions, Department of Energy Grant DE-FG03-86ER13498 (RPG) the UCLA-DOE Institute of Genomics and Proteomics Grant DE-FC02-02ER63421 (RPG), and the National Science Foundation grant NSF 1244566 (MJM and RPG) for methanogen biochemistry.

Publisher Copyright:
© 2016 Gunsalus et al.

Keywords

Anaerobic
Formate
Hydrogen
Methangenic archaea
Methanomicrobiales
Motile
Syntrophic partnerships

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Gunsalus, R. P., Cook, L. E., Crable, B., Rohlin, L., McDonald, E., Mouttaki, H., Sieber, J. R., Poweleit, N., Zhou, H., Lapidus, A. L., Daligault, H. E., Land, M., Gilna, P., Ivanova, N., Kyrpides, N., Culley, D. E., & McInerney, M. J. (2016). Complete genome sequence of Methanospirillum hungatei type strain JF1. Standards in Genomic Sciences, 11(1), Article 2. https://doi.org/10.1186/s40793-015-0124-8

Gunsalus, RP, Cook, LE, Crable, B, Rohlin, L, McDonald, E, Mouttaki, H, Sieber, JR, Poweleit, N, Zhou, H, Lapidus, AL, Daligault, HE, Land, M, Gilna, P, Ivanova, N, Kyrpides, N, Culley, DE & McInerney, MJ 2016, 'Complete genome sequence of Methanospirillum hungatei type strain JF1', Standards in Genomic Sciences, vol. 11, no. 1, 2. https://doi.org/10.1186/s40793-015-0124-8

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abstract = "Methanospirillum hungatei strain JF1 (DSM 864) is a methane-producing archaeon and is the type species of the genus Methanospirillum, which belongs to the family Methanospirillaceae within the order Methanomicrobiales. Its genome was selected for sequencing due to its ability to utilize hydrogen and carbon dioxide and/or formate as a sole source of energy. Ecologically, M. hungatei functions as the hydrogen- and/or formate-using partner with many species of syntrophic bacteria. Its morphology is distinct from other methanogens with the ability to form long chains of cells (up to 100 μm in length), which are enclosed within a sheath-like structure, and terminal cells with polar flagella. The genome of M. hungatei strain JF1 is the first completely sequenced genome of the family Methanospirillaceae, and it has a circular genome of 3,544,738bp containing 3,239 protein coding and 68 RNA genes. The large genome of M. hungatei JF1 suggests the presence of unrecognized biochemical/physiological properties that likely extend to the other Methanospirillaceae and include the ability to form the unusual sheath-like structure and to successfully interact with syntrophic bacteria.",

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Complete genome sequence of Methanospirillum hungatei type strain JF1

Abstract

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