Controlling the contents of microdroplets by exploiting the permeability of PDMS

Jung Uk Shim; Santoshkumar N. Patil; James T. Hodgkinson; Steven D. Bowden; David R. Spring; Martin Welch; Wilhelm T.S. Huck; Florian Hollfelder; Chris Abell

doi:10.1039/c0lc00615g

Controlling the contents of microdroplets by exploiting the permeability of PDMS

Jung Uk Shim, Santoshkumar N. Patil, James T. Hodgkinson, Steven D. Bowden, David R. Spring, Martin Welch, Wilhelm T.S. Huck, Florian Hollfelder, Chris Abell

Food Science and Nutrition

Research output: Contribution to journal › Article › peer-review

32 Scopus citations

Abstract

A microfluidic device capable of exploiting the permeability of small molecules through polydimethylsiloxane (PDMS) has been fabricated in order to control the contents of microdroplets stored in storage wells. We demonstrate that protein precipitation and crystallization can be triggered by delivery of ethanol from a reservoir channel, thus controlling the protein solubility in microdroplets. Likewise quorum sensing in bacteria was triggered by delivery of the auto-inducer N-(3-oxododecanoyl)-l-homoserine lactone (OdDHL) through the PDMS membrane of the device.

Original language	English (US)
Pages (from-to)	1132-1137
Number of pages	6
Journal	Lab on a chip
Volume	11
Issue number	6
DOIs	https://doi.org/10.1039/c0lc00615g
State	Published - Mar 21 2011

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AU - Huck, Wilhelm T.S.

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Controlling the contents of microdroplets by exploiting the permeability of PDMS

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