CRISPR/Cas9-Based Positive Screens for Cancer-Related Traits

Nicholas J. Slipek; Jyotika Varshney; David A. Largaespada

doi:10.1007/978-1-4939-8967-6_11

CRISPR/Cas9-Based Positive Screens for Cancer-Related Traits

Nicholas J. Slipek, Jyotika Varshney, David A. Largaespada

Pediatrics - Hematology

Research output: Chapter in Book/Report/Conference proceeding › Chapter

4 Scopus citations

Abstract

Since the advent of large-scale, detailed descriptive cancer genomics studies at the beginning of the century, such as The Cancer Genome Atlas (TCGA), labs around the world have been working to make this data useful. Data like these can be made more useful by comparison with complementary functional genomic data. One new example is the application of CRISPR/Cas9-based library screening for cancer-related traits in cell lines. Such screens can reveal genome-wide suppressors of tumorigenesis and metastasis. Here we describe the use of widely available lentiviral libraries for such screens in cultured cell lines.

Original language	English (US)
Title of host publication	Methods in Molecular Biology
Publisher	Humana Press Inc.
Pages	137-144
Number of pages	8
DOIs	https://doi.org/10.1007/978-1-4939-8967-6_11
State	Published - 2019

Publication series

Name	Methods in Molecular Biology
Volume	1907
ISSN (Print)	1064-3745
ISSN (Electronic)	1940-6029

Bibliographical note

Publisher Copyright:
© 2019, Springer Science+Business Media, LLC, part of Springer Nature.

Keywords

CRISPR/Cas9
Cancer
Genetic screen
Lentiviral library
Transformation

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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KW - Transformation

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CRISPR/Cas9-Based Positive Screens for Cancer-Related Traits

Abstract

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Bibliographical note

Keywords

UN SDGs

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