Cryptosporidium galli and novel Cryptosporidium avian genotype VI in North American red-winged blackbirds (Agelaius phoeniceus)

Jeba Jesudoss Chelladurai; Mark E. Clark; Martin Kváč; Nikola Holubová; Eakalak Khan; Brianna L.S. Stenger; Catherine W. Giddings; John McEvoy

doi:10.1007/s00436-016-4930-8

Cryptosporidium galli and novel Cryptosporidium avian genotype VI in North American red-winged blackbirds (Agelaius phoeniceus)

Jeba Jesudoss Chelladurai, Mark E. Clark, Martin Kváč, Nikola Holubová, Eakalak Khan, Brianna L.S. Stenger, Catherine W. Giddings, John McEvoy

Research output: Contribution to journal › Article › peer-review

23 Scopus citations

Abstract

Proventriculus and intestinal samples from 70 North American red-winged blackbirds (Agelaius phoeniceus; order Passeriformes) were examined for the presence of Cryptosporidium by PCR amplification and sequence analysis of the 18S ribosomal RNA (18S rRNA), actin, and 70-kDa heat shock protein (HSP70) genes. Twelve birds (17.1 %) were positive for the Cryptosporidium 18S rRNA gene: six birds were positive at the proventriculus site only and six birds were positive at the proventriculus and intestinal sites. Sequence analysis of the 18S rRNA, actin and HSP70 genes showed the presence of the gastric species Cryptosporidium galli in a single proventriculus sample and a closely related genotype, which we have named Cryptosporidium avian genotype VI, in all other positive samples. These findings contribute to our understanding of Cryptosporidium diversification in passerines, the largest avian order.

Original language	English (US)
Pages (from-to)	1901-1906
Number of pages	6
Journal	Parasitology Research
Volume	115
Issue number	5
DOIs	https://doi.org/10.1007/s00436-016-4930-8
State	Published - May 1 2016
Externally published	Yes

Bibliographical note

Funding Information:
The authors gratefully acknowledge support from USDA National Institute of Food and Agriculture (project number: 2008-35102-19260).

Publisher Copyright:
© 2016, Springer-Verlag Berlin Heidelberg.

Keywords

Avian genotype VI
Cryptosporidium
Cryptosporidium galli
Intestine
Passerines
Proventriculus
Red-winged blackbird

Access

10.1007/s00436-016-4930-8

OpenUrl availability

Full text

Cite this

@article{2ddd2eeb0b764442bb68fb48f366f4a8,

title = " Cryptosporidium galli and novel Cryptosporidium avian genotype VI in North American red-winged blackbirds (Agelaius phoeniceus)",

abstract = "Proventriculus and intestinal samples from 70 North American red-winged blackbirds (Agelaius phoeniceus; order Passeriformes) were examined for the presence of Cryptosporidium by PCR amplification and sequence analysis of the 18S ribosomal RNA (18S rRNA), actin, and 70-kDa heat shock protein (HSP70) genes. Twelve birds (17.1 %) were positive for the Cryptosporidium 18S rRNA gene: six birds were positive at the proventriculus site only and six birds were positive at the proventriculus and intestinal sites. Sequence analysis of the 18S rRNA, actin and HSP70 genes showed the presence of the gastric species Cryptosporidium galli in a single proventriculus sample and a closely related genotype, which we have named Cryptosporidium avian genotype VI, in all other positive samples. These findings contribute to our understanding of Cryptosporidium diversification in passerines, the largest avian order.",

keywords = "Avian genotype VI, Cryptosporidium, Cryptosporidium galli, Intestine, Passerines, Proventriculus, Red-winged blackbird",

author = "Chelladurai, {Jeba Jesudoss} and Clark, {Mark E.} and Martin Kv{\'a}{\v c} and Nikola Holubov{\'a} and Eakalak Khan and Stenger, {Brianna L.S.} and Giddings, {Catherine W.} and John McEvoy",

note = "Funding Information: The authors gratefully acknowledge support from USDA National Institute of Food and Agriculture (project number: 2008-35102-19260). Publisher Copyright: {\textcopyright} 2016, Springer-Verlag Berlin Heidelberg.",

year = "2016",

month = may,

day = "1",

doi = "10.1007/s00436-016-4930-8",

language = "English (US)",

volume = "115",

pages = "1901--1906",

journal = "Parasitology Research",

issn = "0932-0113",

publisher = "Springer Verlag",

number = "5",

}

TY - JOUR

T1 - Cryptosporidium galli and novel Cryptosporidium avian genotype VI in North American red-winged blackbirds (Agelaius phoeniceus)

AU - Chelladurai, Jeba Jesudoss

AU - Clark, Mark E.

AU - Kváč, Martin

AU - Holubová, Nikola

AU - Khan, Eakalak

AU - Stenger, Brianna L.S.

AU - Giddings, Catherine W.

AU - McEvoy, John

N1 - Funding Information: The authors gratefully acknowledge support from USDA National Institute of Food and Agriculture (project number: 2008-35102-19260). Publisher Copyright: © 2016, Springer-Verlag Berlin Heidelberg.

PY - 2016/5/1

Y1 - 2016/5/1

N2 - Proventriculus and intestinal samples from 70 North American red-winged blackbirds (Agelaius phoeniceus; order Passeriformes) were examined for the presence of Cryptosporidium by PCR amplification and sequence analysis of the 18S ribosomal RNA (18S rRNA), actin, and 70-kDa heat shock protein (HSP70) genes. Twelve birds (17.1 %) were positive for the Cryptosporidium 18S rRNA gene: six birds were positive at the proventriculus site only and six birds were positive at the proventriculus and intestinal sites. Sequence analysis of the 18S rRNA, actin and HSP70 genes showed the presence of the gastric species Cryptosporidium galli in a single proventriculus sample and a closely related genotype, which we have named Cryptosporidium avian genotype VI, in all other positive samples. These findings contribute to our understanding of Cryptosporidium diversification in passerines, the largest avian order.

AB - Proventriculus and intestinal samples from 70 North American red-winged blackbirds (Agelaius phoeniceus; order Passeriformes) were examined for the presence of Cryptosporidium by PCR amplification and sequence analysis of the 18S ribosomal RNA (18S rRNA), actin, and 70-kDa heat shock protein (HSP70) genes. Twelve birds (17.1 %) were positive for the Cryptosporidium 18S rRNA gene: six birds were positive at the proventriculus site only and six birds were positive at the proventriculus and intestinal sites. Sequence analysis of the 18S rRNA, actin and HSP70 genes showed the presence of the gastric species Cryptosporidium galli in a single proventriculus sample and a closely related genotype, which we have named Cryptosporidium avian genotype VI, in all other positive samples. These findings contribute to our understanding of Cryptosporidium diversification in passerines, the largest avian order.

KW - Avian genotype VI

KW - Cryptosporidium

KW - Cryptosporidium galli

KW - Intestine

KW - Passerines

KW - Proventriculus

KW - Red-winged blackbird

UR - http://www.scopus.com/inward/record.url?scp=84955611877&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84955611877&partnerID=8YFLogxK

U2 - 10.1007/s00436-016-4930-8

DO - 10.1007/s00436-016-4930-8

M3 - Article

C2 - 26818945

AN - SCOPUS:84955611877

SN - 0932-0113

VL - 115

SP - 1901

EP - 1906

JO - Parasitology Research

JF - Parasitology Research

IS - 5

ER -

Cryptosporidium galli and novel Cryptosporidium avian genotype VI in North American red-winged blackbirds (Agelaius phoeniceus)

Abstract

Bibliographical note

Keywords

Access

OpenUrl availability

Other files and links

Fingerprint

Cite this