Crystal structures of ryanodine receptor SPRY1 and tandem-repeat domains reveal a critical FKBP12 binding determinant

Zhiguang Yuchi; Siobhan M Wong King Yuen; Kelvin Lau; Ainsley Q. Underhill; Razvan L. Cornea; James D. Fessenden; Filip Van Petegem

doi:10.1038/ncomms8947

Crystal structures of ryanodine receptor SPRY1 and tandem-repeat domains reveal a critical FKBP12 binding determinant

Zhiguang Yuchi, Siobhan M Wong King Yuen, Kelvin Lau, Ainsley Q. Underhill, Razvan L. Cornea, James D. Fessenden, Filip Van Petegem

Biochemistry, Molecular Biology, and Biophysics (TMED)

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Abstract

Ryanodine receptors (RyRs) form calcium release channels located in the membranes of the sarcoplasmic and endoplasmic reticulum. RyRs play a major role in excitation-contraction coupling and other Ca²⁺-dependent signalling events, and consist of several globular domains that together form a large assembly. Here we describe the crystal structures of the SPRY1 and tandem-repeat domains at 1.2-1.5 Å resolution, which reveal several structural elements not detected in recent cryo-EM reconstructions of RyRs. The cryo-EM studies disagree on the position of SPRY domains, which had been proposed based on homology modelling. Computational docking of the crystal structures, combined with FRET studies, show that the SPRY1 domain is located next to FK506-binding protein (FKBP). Molecular dynamics flexible fitting and mutagenesis experiments suggest a hydrophobic cluster within SPRY1 that is crucial for FKBP binding. A RyR1 disease mutation, N760D, appears to directly impact FKBP binding through interfering with SPRY1 folding.

Original language	English (US)
Article number	7947
Journal	Nature communications
Volume	6
DOIs	https://doi.org/10.1038/ncomms8947
State	Published - Aug 6 2015

Bibliographical note

Funding Information:
We thank the support staff at the Advanced Photon Source (Chicago) GM/CA-CAT beamline 23-ID-D, the Stanford Synchrotron Radiation Light source (Menlo Park, USA), and at the Canadian Light Source (Saskatoon, SK, Canada), which is supported by the Natural Sciences and Engineering Research Council of Canada, the National Research Council Canada, the Canadian Institutes of Health Research (CIHR), the Province of Saskatchewan, Western Economic Diversification Canada, and the University of Saskatchewan. The trilateration software was kindly provided by Dr Bengt Svensson (svens005@umn.edu). This work is supported by an operating grant from the CIHR (MOP-119608, application # 259009) to F.V.P., and NIH grants R01AR059124 (to J.D.F.) and R01HL092097 (to R.L.C.).

Publisher Copyright:
© 2015 Macmillan Publishers Limited. All rights reserved.

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title = "Crystal structures of ryanodine receptor SPRY1 and tandem-repeat domains reveal a critical FKBP12 binding determinant",

abstract = "Ryanodine receptors (RyRs) form calcium release channels located in the membranes of the sarcoplasmic and endoplasmic reticulum. RyRs play a major role in excitation-contraction coupling and other Ca2+-dependent signalling events, and consist of several globular domains that together form a large assembly. Here we describe the crystal structures of the SPRY1 and tandem-repeat domains at 1.2-1.5 {\AA} resolution, which reveal several structural elements not detected in recent cryo-EM reconstructions of RyRs. The cryo-EM studies disagree on the position of SPRY domains, which had been proposed based on homology modelling. Computational docking of the crystal structures, combined with FRET studies, show that the SPRY1 domain is located next to FK506-binding protein (FKBP). Molecular dynamics flexible fitting and mutagenesis experiments suggest a hydrophobic cluster within SPRY1 that is crucial for FKBP binding. A RyR1 disease mutation, N760D, appears to directly impact FKBP binding through interfering with SPRY1 folding.",

author = "Zhiguang Yuchi and Yuen, {Siobhan M Wong King} and Kelvin Lau and Underhill, {Ainsley Q.} and Cornea, {Razvan L.} and Fessenden, {James D.} and {Van Petegem}, Filip",

note = "Funding Information: We thank the support staff at the Advanced Photon Source (Chicago) GM/CA-CAT beamline 23-ID-D, the Stanford Synchrotron Radiation Light source (Menlo Park, USA), and at the Canadian Light Source (Saskatoon, SK, Canada), which is supported by the Natural Sciences and Engineering Research Council of Canada, the National Research Council Canada, the Canadian Institutes of Health Research (CIHR), the Province of Saskatchewan, Western Economic Diversification Canada, and the University of Saskatchewan. The trilateration software was kindly provided by Dr Bengt Svensson (svens005@umn.edu). This work is supported by an operating grant from the CIHR (MOP-119608, application # 259009) to F.V.P., and NIH grants R01AR059124 (to J.D.F.) and R01HL092097 (to R.L.C.). Publisher Copyright: {\textcopyright} 2015 Macmillan Publishers Limited. All rights reserved.",

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Crystal structures of ryanodine receptor SPRY1 and tandem-repeat domains reveal a critical FKBP12 binding determinant

Abstract

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