Abstract
The cardiac muscle isoform of the ryanodine receptor/Ca2+ release channel (RYR) has been proposed to be an important target of cyclic ADP-ribose (cADPR) action in mammalian cells. However, we now demonstrate that neither cADPR (0.1-5 μM), nor the related metabolites, β-NAD+ (0.1-30 mM) and ADP-ribose (0.1-5 μM), affected cardiac RYR activity as determined by [3H]ryanodine binding to cardiac sarcoplasmic reticulum (SR) vesicles. Similarly, cADPR (1 μM) failed to activate single cardiac RYR channels in planar lipid bilayers. Skeletal muscle SR [3H]ryanodine binding was also unaffected by cADPR (up to 30 μM). These results argue against a direct role for the well-characterized RYRs of cardiac or skeletal muscle in mediating cADPR-activated Ca2+ release.
Original language | English (US) |
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Pages (from-to) | 123-126 |
Number of pages | 4 |
Journal | FEBS Letters |
Volume | 352 |
Issue number | 2 |
DOIs | |
State | Published - Sep 26 1994 |
Bibliographical note
Copyright:Copyright 2014 Elsevier B.V., All rights reserved.
Keywords
- Ca release channel
- Cyclic ADP-ribose
- Ryanodine receptor
- Sarcoplasmic reticulum