Decellularization of intact lung tissue through vasculature and airways using negative and positive pressure

Steven Skolasinski; Angela Panoskaltsis-Mortari

doi:10.1007/7651_2017_32

Decellularization of intact lung tissue through vasculature and airways using negative and positive pressure

Steven Skolasinski, Angela Panoskaltsis-Mortari

Research output: Chapter in Book/Report/Conference proceeding › Chapter

4 Scopus citations

Abstract

Decellularization allows the production of extracellular matrix (ECM) scaffolds. Here we describe the use of combined positive pressure and negative pressure to drive decellularization reagents into the vasculature and airways, respectively, of structurally intact lungs in order to remove cells and cellular material leaving an intact ECM scaffold.

Original language	English (US)
Title of host publication	Methods in Molecular Biology
Publisher	Humana Press Inc.
Pages	307-315
Number of pages	9
DOIs	https://doi.org/10.1007/7651_2017_32
State	Published - 2018

Publication series

Name	Methods in Molecular Biology
Volume	1577
ISSN (Print)	1064-3745

Bibliographical note

Publisher Copyright:
© Springer Science+Business Media New York 2017.

Keywords

Decellularization
Lung
Negative pressure bioreactor
Organ scaffold

Access

10.1007/7651_2017_32

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5895527

OpenUrl availability

Full text

Cite this

@inbook{8d9ae604ed234b13b1a8e6b3ecdea58d,

title = "Decellularization of intact lung tissue through vasculature and airways using negative and positive pressure",

abstract = "Decellularization allows the production of extracellular matrix (ECM) scaffolds. Here we describe the use of combined positive pressure and negative pressure to drive decellularization reagents into the vasculature and airways, respectively, of structurally intact lungs in order to remove cells and cellular material leaving an intact ECM scaffold.",

keywords = "Decellularization, Lung, Negative pressure bioreactor, Organ scaffold",

author = "Steven Skolasinski and Angela Panoskaltsis-Mortari",

note = "Publisher Copyright: {\textcopyright} Springer Science+Business Media New York 2017.",

year = "2018",

doi = "10.1007/7651_2017_32",

language = "English (US)",

series = "Methods in Molecular Biology",

publisher = "Humana Press Inc.",

pages = "307--315",

booktitle = "Methods in Molecular Biology",

}

TY - CHAP

T1 - Decellularization of intact lung tissue through vasculature and airways using negative and positive pressure

AU - Skolasinski, Steven

AU - Panoskaltsis-Mortari, Angela

N1 - Publisher Copyright: © Springer Science+Business Media New York 2017.

PY - 2018

Y1 - 2018

N2 - Decellularization allows the production of extracellular matrix (ECM) scaffolds. Here we describe the use of combined positive pressure and negative pressure to drive decellularization reagents into the vasculature and airways, respectively, of structurally intact lungs in order to remove cells and cellular material leaving an intact ECM scaffold.

AB - Decellularization allows the production of extracellular matrix (ECM) scaffolds. Here we describe the use of combined positive pressure and negative pressure to drive decellularization reagents into the vasculature and airways, respectively, of structurally intact lungs in order to remove cells and cellular material leaving an intact ECM scaffold.

KW - Decellularization

KW - Lung

KW - Negative pressure bioreactor

KW - Organ scaffold

UR - http://www.scopus.com/inward/record.url?scp=85057541351&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85057541351&partnerID=8YFLogxK

U2 - 10.1007/7651_2017_32

DO - 10.1007/7651_2017_32

M3 - Chapter

C2 - 28656583

AN - SCOPUS:85057541351

T3 - Methods in Molecular Biology

SP - 307

EP - 315

BT - Methods in Molecular Biology

PB - Humana Press Inc.

ER -

Decellularization of intact lung tissue through vasculature and airways using negative and positive pressure

Abstract

Publication series

Bibliographical note

Keywords

Access

OpenUrl availability

Other files and links

Fingerprint

Cite this