TY - JOUR
T1 - Deep eutectic solvents for Candida antarctica lipase B-catalyzed reactions
AU - Gorke, Johnathan T.
AU - Srienc, Friedrich
AU - Kazlauskas, Romas J.
PY - 2010/4/28
Y1 - 2010/4/28
N2 - Many deep eutectic solvents (DES's) are mixtures of ammonium salts and hydrogen-bond donors; e.g., a 1:2 mixture of choline chloride and urea. Like room temperature ionic liquids, DES's are polar, viscous solvents with low vapor pressure and flammability. However, synthesis of DES's is simpler - requiring only mixing of components - and the components are non-toxic and approximately ten-fold less expensive than the components for ionic liquids. Even though components of DES's can include protein denaturants like urea, we found that immobilized Candida antarctica lipase B retains activity in a wide range of DES's. Rates of transesterification, aminolysis of esters, perhydrolysis were similar to those in organic solvents and several-fold faster than those in ionic liquids. In a few cases, side reactions with the components of the DES occurred. DES's containing sugars were highly viscous and required temperatures ≥60 °C to permit stirring.
AB - Many deep eutectic solvents (DES's) are mixtures of ammonium salts and hydrogen-bond donors; e.g., a 1:2 mixture of choline chloride and urea. Like room temperature ionic liquids, DES's are polar, viscous solvents with low vapor pressure and flammability. However, synthesis of DES's is simpler - requiring only mixing of components - and the components are non-toxic and approximately ten-fold less expensive than the components for ionic liquids. Even though components of DES's can include protein denaturants like urea, we found that immobilized Candida antarctica lipase B retains activity in a wide range of DES's. Rates of transesterification, aminolysis of esters, perhydrolysis were similar to those in organic solvents and several-fold faster than those in ionic liquids. In a few cases, side reactions with the components of the DES occurred. DES's containing sugars were highly viscous and required temperatures ≥60 °C to permit stirring.
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U2 - 10.1021/bk-2010-1038.ch014
DO - 10.1021/bk-2010-1038.ch014
M3 - Article
AN - SCOPUS:84898453399
SN - 0097-6156
VL - 1038
SP - 169
EP - 180
JO - ACS Symposium Series
JF - ACS Symposium Series
ER -