Abstract
The present study aimed to establish an early model of the malting barley transcriptome, which describes the expression of genes and their ontologies, identify the period during malting with the largest dynamic shift in gene expression for future investigation, and to determine the expression patterns of all starch degrading enzyme genes relevant to the malting and brewing industry. Large dynamic increases in gene expression occurred early in malting with differential expressed genes enriched for cell wall and starch hydrolases amongst many malting related categories. Twenty-five of forty starch degrading enzyme genes were differentially expressed in the malting barley transcriptome including eleven α-amylase genes, six β-amylase genes, three α-glucosidase genes, and all five starch debranching enzyme genes. Four new or novel α-amylase genes, one β-amylase gene (Bmy3), three α-glucosidase genes, and two isoamylase genes had appreciable expression that requires further exploration into their potential relevance to the malting and brewing industry.
Original language | English (US) |
---|---|
Pages (from-to) | 3310-3324 |
Number of pages | 15 |
Journal | Genomics |
Volume | 113 |
Issue number | 5 |
DOIs | |
State | Published - Sep 2021 |
Bibliographical note
Funding Information:We thank Michael O'Connor for his excellent technical assistance, Colin Cassidy for his technical assistance, and Chris Martens for malting assistance. Research supported by U.S. Department of Agriculture-Agricultural Research Service. The U.S. Department of Agriculture is an equal opportunity provider and employer. Mention of trade names or commercial products in this publication is solely for the purpose of providing specific information and does not imply recommendation or endorsement by the U.S. Department of Agriculture.
Funding Information:
Research supported by U.S. Department of Agriculture - Agricultural Research Service . The U.S. Department of Agriculture is an equal opportunity provider and employer. Mention of trade names or commercial products in this publication is solely for the purpose of providing specific information and does not imply recommendation or endorsement by the U.S. Department of Agriculture.
Publisher Copyright:
© 2021
Keywords
- Alpha-amylase
- Alpha-glucosidase
- Beta-amylase
- Germination
- Isoamylase
- Limit dextrinase
- Starch degradation