Design and use of constitutively active STAT5 constructs

Michael A. Farrar

doi:10.1016/B978-0-12-381296-4.00030-0

Design and use of constitutively active STAT5 constructs

Michael A. Farrar

Laboratory Medicine and Pathology

Research output: Chapter in Book/Report/Conference proceeding › Chapter

6 Scopus citations

Abstract

The transcription factor Signal Tranducer and Activator of Transcription 5 (STAT5) plays an important role in numerous biological processes including, but not limited to, (i) homeostasis of hematopoietic stem cells, (ii) development of essentially all blood cell lineages, (iii) growth hormone effects, (iv) differentiation of mammary epithelium, and (v) central nervous system control of metabolism. Two key tools for deciphering STAT5 biology have involved the use of mice in which the Stat5a and Stat5b genes can be conditionally deleted (Stat5 ^FL/FL mice) and the development of systems in which STAT5a or STAT5b is rendered constitutively active. In this chapter, the distinct mechanisms that have been developed to render STAT5 constitutively active and their use in probing biological processes are discussed.

Original language	English (US)
Title of host publication	Methods in Enzymology
Publisher	Academic Press Inc.
Pages	583-596
Number of pages	14
Edition	C
DOIs	https://doi.org/10.1016/B978-0-12-381296-4.00030-0
State	Published - 2010

Publication series

Name	Methods in Enzymology
Number	C
Volume	485
ISSN (Print)	0076-6879

Bibliographical note

Funding Information:
I thank Dr Lynn Heltemes Harris for critical review of this manuscript. I am currently supported by a Leukemia and Lymphoma Society Scholar award.

Access

10.1016/B978-0-12-381296-4.00030-0

OpenUrl availability

Full text

Cite this

@inbook{33a911f066a54bb1acc0e231625ab580,

title = "Design and use of constitutively active STAT5 constructs",

abstract = " The transcription factor Signal Tranducer and Activator of Transcription 5 (STAT5) plays an important role in numerous biological processes including, but not limited to, (i) homeostasis of hematopoietic stem cells, (ii) development of essentially all blood cell lineages, (iii) growth hormone effects, (iv) differentiation of mammary epithelium, and (v) central nervous system control of metabolism. Two key tools for deciphering STAT5 biology have involved the use of mice in which the Stat5a and Stat5b genes can be conditionally deleted (Stat5 FL/FL mice) and the development of systems in which STAT5a or STAT5b is rendered constitutively active. In this chapter, the distinct mechanisms that have been developed to render STAT5 constitutively active and their use in probing biological processes are discussed.",

author = "Farrar, {Michael A.}",

note = "Funding Information: I thank Dr Lynn Heltemes Harris for critical review of this manuscript. I am currently supported by a Leukemia and Lymphoma Society Scholar award.",

year = "2010",

doi = "10.1016/B978-0-12-381296-4.00030-0",

language = "English (US)",

series = "Methods in Enzymology",

publisher = "Academic Press Inc.",

number = "C",

pages = "583--596",

booktitle = "Methods in Enzymology",

address = "United States",

edition = "C",

}

TY - CHAP

T1 - Design and use of constitutively active STAT5 constructs

AU - Farrar, Michael A.

N1 - Funding Information: I thank Dr Lynn Heltemes Harris for critical review of this manuscript. I am currently supported by a Leukemia and Lymphoma Society Scholar award.

PY - 2010

Y1 - 2010

N2 - The transcription factor Signal Tranducer and Activator of Transcription 5 (STAT5) plays an important role in numerous biological processes including, but not limited to, (i) homeostasis of hematopoietic stem cells, (ii) development of essentially all blood cell lineages, (iii) growth hormone effects, (iv) differentiation of mammary epithelium, and (v) central nervous system control of metabolism. Two key tools for deciphering STAT5 biology have involved the use of mice in which the Stat5a and Stat5b genes can be conditionally deleted (Stat5 FL/FL mice) and the development of systems in which STAT5a or STAT5b is rendered constitutively active. In this chapter, the distinct mechanisms that have been developed to render STAT5 constitutively active and their use in probing biological processes are discussed.

AB - The transcription factor Signal Tranducer and Activator of Transcription 5 (STAT5) plays an important role in numerous biological processes including, but not limited to, (i) homeostasis of hematopoietic stem cells, (ii) development of essentially all blood cell lineages, (iii) growth hormone effects, (iv) differentiation of mammary epithelium, and (v) central nervous system control of metabolism. Two key tools for deciphering STAT5 biology have involved the use of mice in which the Stat5a and Stat5b genes can be conditionally deleted (Stat5 FL/FL mice) and the development of systems in which STAT5a or STAT5b is rendered constitutively active. In this chapter, the distinct mechanisms that have been developed to render STAT5 constitutively active and their use in probing biological processes are discussed.

UR - http://www.scopus.com/inward/record.url?scp=78049516990&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=78049516990&partnerID=8YFLogxK

U2 - 10.1016/B978-0-12-381296-4.00030-0

DO - 10.1016/B978-0-12-381296-4.00030-0

M3 - Chapter

C2 - 21050937

AN - SCOPUS:78049516990

T3 - Methods in Enzymology

SP - 583

EP - 596

BT - Methods in Enzymology

PB - Academic Press Inc.

ER -

Design and use of constitutively active STAT5 constructs

Abstract

Publication series

Bibliographical note

Access

OpenUrl availability

Other files and links

Fingerprint

Cite this