TY - JOUR
T1 - Detection and serotyping of dengue viruses by PCR
T2 - a simple, rapid method for the isolation of viral RNA from infected mosquito larvae.
AU - Chan, S. Y.
AU - Kautner, I.
AU - Lam, S. K.
PY - 1994/6/1
Y1 - 1994/6/1
N2 - Dengue viruses pose a considerable global public health problem with an estimated 100 million cases of illness every year. This illustrates the need for rapid and reliable diagnostic methods for proper patient management and disease control. Currently, laboratory diagnosis depends on serology or virus isolation, with both methods having certain drawbacks. Alternatively, reverse transcription and polymerase chain reaction (RT-PCR) offers the potential for the rapid, highly sensitive and specific detection of dengue viruses. Since we occasionally encounter the problem of insufficient amounts of patient serum for the direct detection of dengue viruses, a method was developed for the extraction of viral RNA after biological amplification in mosquito larvae. Using this method, 15 of 19 clinical samples tested were correctly identified using RT-PCR.
AB - Dengue viruses pose a considerable global public health problem with an estimated 100 million cases of illness every year. This illustrates the need for rapid and reliable diagnostic methods for proper patient management and disease control. Currently, laboratory diagnosis depends on serology or virus isolation, with both methods having certain drawbacks. Alternatively, reverse transcription and polymerase chain reaction (RT-PCR) offers the potential for the rapid, highly sensitive and specific detection of dengue viruses. Since we occasionally encounter the problem of insufficient amounts of patient serum for the direct detection of dengue viruses, a method was developed for the extraction of viral RNA after biological amplification in mosquito larvae. Using this method, 15 of 19 clinical samples tested were correctly identified using RT-PCR.
UR - http://www.scopus.com/inward/record.url?scp=0028458471&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0028458471&partnerID=8YFLogxK
M3 - Article
C2 - 7855637
AN - SCOPUS:0028458471
SN - 0125-1562
VL - 25
SP - 258
EP - 261
JO - The Southeast Asian journal of tropical medicine and public health
JF - The Southeast Asian journal of tropical medicine and public health
IS - 2
ER -