Detection and Sourcing of Gluten in Grain with Multiple Floating-Gate Transistor Biosensors

Scott P. White; C. Daniel Frisbie; Kevin D. Dorfman

doi:10.1021/acssensors.7b00810

Detection and Sourcing of Gluten in Grain with Multiple Floating-Gate Transistor Biosensors

Scott P. White, C. Daniel Frisbie, Kevin D. Dorfman

Chemical Engineering and Materials Science

Research output: Contribution to journal › Article › peer-review

32 Scopus citations

Abstract

We report a chemically tunable electronic sensor for quantitation of gluten based on a floating-gate transistor (FGT) architecture. The FGTs are fabricated in parallel and each one is functionalized with a different chemical moiety designed to preferentially bind a specific grain source of gluten. The resulting set of FGT sensors can detect both wheat and barley gluten below the gluten-free limit of 20 ppm (w/w) while providing a source-dependent signature for improved accuracy. This label-free transduction method does not require any secondary binding events, resulting in a ca. 45 min reduction in analysis time relative to state-of-the-art ELISA kits with a simple and easily implemented workflow.

Original language	English (US)
Pages (from-to)	395-402
Number of pages	8
Journal	ACS Sensors
Volume	3
Issue number	2
DOIs	https://doi.org/10.1021/acssensors.7b00810
State	Published - Feb 23 2018

Bibliographical note

Funding Information:
We acknowledge funding from the National Science Foundation (Predoctoral Fellowship for SPW), the University of Minnesota MN-Drive program, and the Office of Naval Research through the Multi-University Research Initiative (CDF). A portion of this work was performed at the University of Minnesota Nanofabrication Center which received partial support from the NSF through NNIN.

Publisher Copyright:
© 2018 American Chemical Society.

Copyright:
Copyright 2018 Elsevier B.V., All rights reserved.

Keywords

biosensor
gluten
label-free
microfluidics
printed electronics

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title = "Detection and Sourcing of Gluten in Grain with Multiple Floating-Gate Transistor Biosensors",

abstract = "We report a chemically tunable electronic sensor for quantitation of gluten based on a floating-gate transistor (FGT) architecture. The FGTs are fabricated in parallel and each one is functionalized with a different chemical moiety designed to preferentially bind a specific grain source of gluten. The resulting set of FGT sensors can detect both wheat and barley gluten below the gluten-free limit of 20 ppm (w/w) while providing a source-dependent signature for improved accuracy. This label-free transduction method does not require any secondary binding events, resulting in a ca. 45 min reduction in analysis time relative to state-of-the-art ELISA kits with a simple and easily implemented workflow.",

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AB - We report a chemically tunable electronic sensor for quantitation of gluten based on a floating-gate transistor (FGT) architecture. The FGTs are fabricated in parallel and each one is functionalized with a different chemical moiety designed to preferentially bind a specific grain source of gluten. The resulting set of FGT sensors can detect both wheat and barley gluten below the gluten-free limit of 20 ppm (w/w) while providing a source-dependent signature for improved accuracy. This label-free transduction method does not require any secondary binding events, resulting in a ca. 45 min reduction in analysis time relative to state-of-the-art ELISA kits with a simple and easily implemented workflow.

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Detection and Sourcing of Gluten in Grain with Multiple Floating-Gate Transistor Biosensors

Abstract

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