Detection of PERK Signaling in the Central Nervous System

Zhixin Lei; Sarrabeth Stone; Wensheng Lin

doi:10.1007/978-1-0716-1732-8_15

Detection of PERK Signaling in the Central Nervous System

Zhixin Lei, Sarrabeth Stone, Wensheng Lin

Neuroscience

Research output: Chapter in Book/Report/Conference proceeding › Chapter

Abstract

In response to endoplasmic reticulum (ER) stress, activation of pancreatic ER kinase (PERK) signaling adapts cells to stressful conditions by phosphorylating eukaryotic translation initiation factor 2α (eIF2α). Phosphorylation of eIF2α inhibits global protein translation but stimulates the expression of numerous stress-responsive genes by inducing the transcription factor ATF4. A large number of studies have shown that activation of PERK signaling has beneficial or detrimental effects in various diseases of the central nervous system (CNS), including neurodegenerative diseases, myelin disorders, CNS injuries, among others. This chapter is devoted to describing the practical methods for the detection of PERK signaling in CNS diseases.

Original language	English (US)
Title of host publication	Methods in Molecular Biology
Publisher	Humana Press Inc.
Pages	233-245
Number of pages	13
DOIs	https://doi.org/10.1007/978-1-0716-1732-8_15
State	Published - 2022

Publication series

Name	Methods in Molecular Biology
Volume	2378
ISSN (Print)	1064-3745
ISSN (Electronic)	1940-6029

Bibliographical note

Publisher Copyright:
© 2022, The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature.

Keywords

ATF4
CHOP
GADD34
Immunofluorescence
Neuron
Oligodendrocyte
PERK
Phosphorylated eIF2α
Real-time PCR
Western blot

PubMed: MeSH publication types

Journal Article
Research Support, N.I.H., Extramural

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10.1007/978-1-0716-1732-8_15

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title = "Detection of PERK Signaling in the Central Nervous System",

abstract = "In response to endoplasmic reticulum (ER) stress, activation of pancreatic ER kinase (PERK) signaling adapts cells to stressful conditions by phosphorylating eukaryotic translation initiation factor 2α (eIF2α). Phosphorylation of eIF2α inhibits global protein translation but stimulates the expression of numerous stress-responsive genes by inducing the transcription factor ATF4. A large number of studies have shown that activation of PERK signaling has beneficial or detrimental effects in various diseases of the central nervous system (CNS), including neurodegenerative diseases, myelin disorders, CNS injuries, among others. This chapter is devoted to describing the practical methods for the detection of PERK signaling in CNS diseases.",

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KW - Neuron

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KW - Real-time PCR

KW - Western blot

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Detection of PERK Signaling in the Central Nervous System

Abstract

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