Abstract
Recent developments in the direct observation of J couplings across hydrogen bonds in proteins and nucleic acids provide additional information for structure and function studies of these molecules by NMR spectroscopy. A J NN -correlated [ 15 N, 1 H] TROSY experiment proposed by Pervushin et al. (Proc. Natl. Acad. Sci. USA 95, 14147-14151, 1998) can be applied to measure h J HN in smaller nucleic acids in an E.COSY manner. However, it cannot be effectively applied to large nucleic acids, such as tRNA Trp , since one of the peaks corresponding to a fast relaxing component will be too weak to be observed in the spectra of large molecules. In this Communication, we proposed a modified J NN -correlated [ 15 N, 1 H] TROSY experiment which enables direct measurement of h J HN in large nucleic acids.
Original language | English (US) |
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Pages (from-to) | 357-360 |
Number of pages | 4 |
Journal | Journal of Magnetic Resonance |
Volume | 147 |
Issue number | 2 |
DOIs | |
State | Published - Dec 2000 |
Bibliographical note
Funding Information:This work is supported by grants from the Research Grant Council of Hong Kong (HKUST6197/97M, HKUST6038/98M, and HKUST6199/99M). The Hong Kong Biotechnology Research Institute is acknowledged for the purchase of the 750-MHz NMR spectrometer. The authors also thank Dr. H. Xue and Prof. J. Wong for their help with sample preparation.
Keywords
- Hydrogen bond
- J -correlated [ N, H] TROSY
- NMR
- TROSY
- tRNA