Development of a multiphoton fluorescence lifetime imaging microscopy system using a streak camera

R. V. Krishnan; H. Saitoh; H. Terada; V. E. Centonze; B. Herman

doi:10.1063/1.1569410

Development of a multiphoton fluorescence lifetime imaging microscopy system using a streak camera

R. V. Krishnan, H. Saitoh, H. Terada, V. E. Centonze, B. Herman

Biomedical Engineering

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Abstract

The development and calibration of a multiphoton fluorescence lifetime imaging microscopy (FLIM) system was described. FLIM studies were carried out on cellular specimens such as stained pollen grains and fibroblast cells. The lifetime values were found to correspond well with results of earlier studies on the same specimens. Measurement of intracellular physiology and fluorescence resonance energy transfer imaging were identified as potential applications for the system.

Original language	English (US)
Pages (from-to)	2714-2721
Number of pages	8
Journal	Review of Scientific Instruments
Volume	74
Issue number	5
DOIs	https://doi.org/10.1063/1.1569410
State	Published - May 2003

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abstract = "The development and calibration of a multiphoton fluorescence lifetime imaging microscopy (FLIM) system was described. FLIM studies were carried out on cellular specimens such as stained pollen grains and fibroblast cells. The lifetime values were found to correspond well with results of earlier studies on the same specimens. Measurement of intracellular physiology and fluorescence resonance energy transfer imaging were identified as potential applications for the system.",

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AU - Herman, B.

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Development of a multiphoton fluorescence lifetime imaging microscopy system using a streak camera

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